Development of a label-free electrochemical aptasensor for Rift Valley fever virus detection.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Rift Valley fever virus*/isolation & purification ; Aptamers, Nucleotide*/chemistry ; Biosensing Techniques*/methods ; Electrochemical Techniques*/methods; SELEX Aptamer Technique ; Rift Valley Fever/diagnosis ; Rift Valley Fever/virology ; Limit of Detection ; Humans ; Gold/chemistry ; Electrodes

In this research, we describe the first aptasensor for the detection of the Rift Valley Fever virus (RVFV). The process involved the selection of aptamers through the systematic evolution of ligands by the exponential enrichment (SELEX) technique. After 12 rounds of selection, 6 aptamers were selected and the corresponding binding affinities were assessed using fluorescence binding assays, revealing dissociation constants ranging from 15.45 to 40.98 nM. Notably, among the aptamers, RV2 and RV3 exhibited the highest binding affinities toward RVFV, with dissociation constants of 15.45 and 18.62 nM, respectively. Thiol-modified aptamers were subsequently immobilized onto screen-printed gold electrodes, facilitating the label-free detection of RVFV through square wave voltammetry. The voltammetric aptasensor demonstrated an excellent sensitivity, with a detection limit of 0.015 ng/mL. In addition, cross-reactivity assessments were conducted, where negligible response was obtained when the aptasensor was exposed to non-specific proteins.
(© 2024. The Author(s).)

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Keywords: Aptamer; Aptasensor; Electrochemistry; Label-free detection; Rift Valley fever virus; SELEX

0 (Aptamers, Nucleotide)
7440-57-5 (Gold)

Date Created: 20241012 Date Completed: 20241012 Latest Revision: 20241015

20241016

PMC11470950

10.1038/s41598-024-74314-7

39396078