Measuring Calcium Levels in Bone-Resorbing Osteoclasts and Bone-Forming Osteoblasts.

Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,

Osteoclasts*/metabolism ; Osteoclasts*/cytology ; Osteoblasts*/metabolism ; Osteoblasts*/cytology ; Calcium*/metabolism ; Cell Differentiation* ; Bone Resorption*/metabolism; Humans ; Osteogenesis ; Cells, Cultured ; Calcium Signaling ; Fura-2/metabolism

Bone remodeling is a crucial, dynamic process that renews bone and maintains mineral homeostasis. It consists of several steps, including osteoclastic bone resorption and osteoblastic bone formation and mineralization. Intracellular calcium signaling is essential for osteoclast and osteoblast differentiation and activity. Here, we describe the differentiation of human osteoclasts and osteoblasts in vitro and provide common methods to determine cell differentiation and activity. We then describe protocols for measuring intracellular calcium in these cells using Fura2-AM.
(© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

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Keywords: Bone formation; Bone mineralization; Bone resorption; Fura-2; Osteoblasts; Osteoclasts

SY7Q814VUP (Calcium)
TSN3DL106G (Fura-2)

Date Created: 20241012 Date Completed: 20241012 Latest Revision: 20241012

20241013

10.1007/978-1-0716-4164-4_13

39395105