Protocol for inducing varying TBI severity in a mouse model using a closed-head, weight-drop, impact-induced acceleration mechanism.

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  • Additional Information
    • Source:
      Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Cambridge, MA] : Cell Press, [2020]-
    • Subject Terms:
      Brain Injuries, Traumatic*/physiopathology ; Brain Injuries, Traumatic*/pathology ; Disease Models, Animal*; Animals ; Mice ; Acceleration ; Male
    • Abstract:
      Animal models of traumatic brain injury (TBI) are critical for understanding its complex neuropathology. Here, we present a protocol to induce varying TBI severities in mice using a closed-head, weight-drop model that includes an impact-induced acceleration mechanism. We describe steps for habituation with neurological severity score (NSS) equipment, assessing NSS baseline, performing anesthesia and TBI, assessing NSS post-injury, and analyzing data. This protocol requires no prior surgical intervention and is adaptable for rat studies. For complete details on the use and execution of this protocol, please refer to PhD Dissertation of Javier Allende Labastida 1 and Tang et al. 2 .
      Competing Interests: Declaration of interests The authors declare no competing interests.
      (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: health sciences; model organisms; neuroscience
    • Publication Date:
      Date Created: 20241011 Date Completed: 20241224 Latest Revision: 20241224
    • Publication Date:
      20241224
    • Accession Number:
      10.1016/j.xpro.2024.103370
    • Accession Number:
      39392749