First molecular investigation to detect avian Mycoplasma species in clinical samples from laying-hen farms in Tunisia.

Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE

Original Publication: London : BioMed Central, 2005-

Mycoplasma Infections*/veterinary ; Mycoplasma Infections*/epidemiology ; Mycoplasma Infections*/microbiology ; Poultry Diseases*/microbiology ; Poultry Diseases*/epidemiology ; Mycoplasma*/isolation & purification ; Mycoplasma*/genetics ; Mycoplasma*/classification ; Chickens*/microbiology ; Polymerase Chain Reaction*/veterinary; Animals ; Tunisia/epidemiology ; Female ; Prevalence

Background: Avian mycoplasmas are known pathogens, which cause severe economic losses in poultry flocks. PCR is a rapid, sensitive, and less expensive diagnostic tool than culture for the identification of mycoplasmas in poultry farms. The objective of this study was to determine by PCR the presence of Mycoplasma spp., Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS), and Mycoplasma pullorum (MP) in laying hens located in the Sfax region, in the South of Tunisia.
Results: A total of 781 tracheal swabs were collected from 13 laying-hen farms without clinical signs at the date of sampling. MP was detected by a newly described specific PCR assay. The prevalence calculated from PCR results at the flock level was 100% for Mycoplasma spp., 0% for MG, 84.6% for MS and 61.5% for MP. The overall prevalence at the animal level was 38.7% for Mycoplasma spp., 0% for MG, 25% for MS and 6.4% for MP. The overall prevalence of 100% of avian mycoplasmas in laying-hen farms (38.7% prevalence at the animal level) shows an alarming situation.
Conclusions: These results underline the importance of monitoring the emergence and spread of Mycoplasma strains in farms in order to decrease economic losses due to mycoplasmoses.
(© 2024. The Author(s).)

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Keywords: Mycoplasma gallisepticum; Mycoplasma synoviae; Mycoplasma pullorum; Hen; PCR

Date Created: 20241010 Date Completed: 20241011 Latest Revision: 20241013

20241013

PMC11465938

10.1186/s12917-024-04311-5

39390571