SUN1 inhibits osteogenesis and promotes adipogenesis of human adipose-derived stem cells by regulating α-tubulin and CD36 expression.

Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101083777 Publication Model: Print Cited Medium: Internet ISSN: 1582-4934 (Electronic) Linking ISSN: 15821838 NLM ISO Abbreviation: J Cell Mol Med Subsets: MEDLINE

Publication: Oxford, England : Wiley-Blackwell
Original Publication: Bucharest : "Carol Davila" University Press, 2000-

Adipogenesis*/genetics ; CD36 Antigens*/metabolism ; CD36 Antigens*/genetics ; Osteogenesis*/genetics ; Tubulin*/metabolism ; Stem Cells*/metabolism ; Stem Cells*/cytology ; PPAR gamma*/metabolism ; PPAR gamma*/genetics ; Cell Differentiation* ; Adipose Tissue*/cytology ; Adipose Tissue*/metabolism; Humans ; Membrane Proteins/metabolism ; Membrane Proteins/genetics ; Microtubule-Associated Proteins/metabolism ; Microtubule-Associated Proteins/genetics ; Gene Expression Regulation ; Cells, Cultured ; Nuclear Proteins

Sad and UNC84 domain 1 (SUN1) is a kind of nuclear envelope protein with established involvement in cellular processes, including nuclear motility and meiosis. SUN1 plays an intriguing role in human adipose-derived stem cells (hASCs) differentiation; however, this role remains largely undefined. This study was undertaken to investigate the role of SUN1 in hASCs differentiation, as well as its underlying mechanisms. Employing siRNAs, we selectively downregulated SUN1 and CD36 expression. Microtubules were depolymerized using nocodazole, and PPARγ was activated using rosiglitazone. Western blotting was performed to quantify SUN1, PPARγ, α-tubulin, CD36, OPN, and adiponectin protein expression levels. Alkaline phosphatase and Oil red O staining were used to assess osteogenesis and adipogenesis, respectively. Downregulated SUN1 expression increased osteogenesis and decreased adipogenesis in hASCs, concomitant with upregulated α-tubulin expression and downregulated CD36 expression, alongside reduced nuclear localization of PPARγ. Microtubule depolymerization increased CD36 expression. Rescue experiments indicated that microtubule depolymerization counteracted the downregulated SUN1-induced phenotypic changes. This study demonstrates that SUN1 influences the differentiation of hASCs towards osteogenic and adipogenic lineages, indicating its essential role in cell fate.
(© 2024 The Author(s). Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.)

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2022YFF1202603 National Key R&D Program of China; 1202103001 President's Foundation of TCM-Integrated Hospital of Southern Medical University

Keywords: CD36; Sad1 and UNC84 domain 1; adipogenesis; osteogenesis; α‐Tubulin

0 (CD36 Antigens)
0 (Tubulin)
0 (PPAR gamma)
0 (SUN1 protein, human)
0 (Membrane Proteins)
0 (Microtubule-Associated Proteins)
0 (CD36 protein, human)
0 (Nuclear Proteins)

Date Created: 20241009 Date Completed: 20241009 Latest Revision: 20241011

20241011

PMC11463318

10.1111/jcmm.70143

39383106