Effect of phosphatase and tensin homolog-induced putative kinase 1/ E3 ubiquitin ligase Parkin mediated mitochondrial autophagy on chronic kidney disease myocardial injury and the intervention mechanism of Shenshuai recipe.

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  • Additional Information
    • Source:
      Publisher: Academy Of Traditional Chinese Medicine Country of Publication: China NLM ID: 8211546 Publication Model: Print Cited Medium: Internet ISSN: 2589-451X (Electronic) Linking ISSN: 02552922 NLM ISO Abbreviation: J Tradit Chin Med Subsets: MEDLINE
    • Publication Information:
      Publication: Beijing : Academy Of Traditional Chinese Medicine
      Original Publication: [Beijing, China : Pub. Office, Journal of Traditional Chinese Medicine, 1981-
    • Subject Terms:
      Drugs, Chinese Herbal*/administration & dosage ; Drugs, Chinese Herbal*/pharmacology ; Renal Insufficiency, Chronic*/drug therapy ; Renal Insufficiency, Chronic*/metabolism ; Renal Insufficiency, Chronic*/genetics ; Renal Insufficiency, Chronic*/physiopathology ; Autophagy*/drug effects ; Ubiquitin-Protein Ligases*/genetics ; Ubiquitin-Protein Ligases*/metabolism ; Rats, Sprague-Dawley* ; Protein Kinases*/metabolism ; Protein Kinases*/genetics; Animals ; Rats ; Male ; Humans ; Mitochondria/drug effects ; Mitochondria/metabolism ; Myocardium/metabolism
    • Abstract:
      Objective: To study whether Shenshuai recipe (, SSR) can play a protective role on chronic kidney disease myocardial injury model through phosphatase and tensin homolog-induced putative kinase 1 (PINK1)/E3 ubiquitin ligase Parkin (Parkin) mitochondrial autophagy pathway.
      Methods: Forty-eight nephrectomized rats were randomly divided into six groups: sham-operated group, model group, Benazepril group, low, medium and high-dose groups of SSR. The rats were given the cor-responding intervention for six weeks, then were sacrificed. Serum was examined by enzyme linked immunosorbent assay (ELISA). Cardiac ultrasound was used to detect cardiac function in 5/6 nephrectomized rats. Myocardial tissue was examined by light and electron microscopy; PINK1, Parkin, microtubule-associated protein1 light chain 3 II (LC3B), sequestosome 1 (P62), BECN1 (Beclin-1) and dynamin-related protein 1 (Drp-1) were measured by real time polymerase chain reaction (RT-PCR), Western blot (WB) and immunohistochemistry (IHC).
      Results: The expression levels of blood urea nitrogen (BUN) and creatinine (SCr) in the model group were significantly higher than those in the sham-operated group, indicating that modeling was successful. SSR can protect myocardium by reducing the relative expression of creatine kinase myocardial isoenzyme and hypersensitivity cardiac troponin I ( P <0.05). SSR can improve cardiac function in rats after ultrasound testing. SSR can improve the pathological manifestations of myocardial tissue after Masson staining. SSR can increase the number of autophagosomes and autophagiclysosomes in 5/6 nephrectomized rats ( P <0.05). Determined by RT-PCR, WB and IHC, SSR can increase the relative expression of PINK1, Parkin, and LC3B ( P <0.05), and decrease the relative expression of P62, Beclin-1 and Drp-1 ( P <0.05).
      Conclusions: The PINK1/Parkin mitochondrial autophagy pathway in myocardial tissues in 5/6 nephrectomy CKD myocardial injury rats was inhibited. SSR can activate PINK1/Parkin mitochondrial autophagy to enhance mitochondrial autophagy, and play a protective role in myocardial tissues.
    • References:
      Can J Physiol Pharmacol. 2014 Jul;92(7):583-91. (PMID: 24905188)
      Cardiovasc Diabetol. 2021 May 7;20(1):100. (PMID: 33962654)
      J Agric Food Chem. 2022 Mar 9;70(9):2923-2935. (PMID: 35195395)
      Cell Death Dis. 2022 Apr 8;13(4):319. (PMID: 35395830)
      J Mol Cell Cardiol. 2022 Apr;165:1-8. (PMID: 34919896)
      Trends Biochem Sci. 2021 Apr;46(4):329-343. (PMID: 33323315)
      Cell Mol Life Sci. 2021 Apr;78(8):3817-3851. (PMID: 33580835)
      BMC Complement Med Ther. 2020 Jul 2;20(1):203. (PMID: 32615967)
      Zhongguo Zhong Yao Za Zhi. 2020 Jun;45(12):2960-2965. (PMID: 32627473)
      Zhongguo Zhong Yao Za Zhi. 2022 Jun;47(11):3066-3072. (PMID: 35718531)
      Pharmacol Res. 2022 Apr;178:106186. (PMID: 35306141)
      Med Mol Morphol. 2022 Mar;55(1):41-46. (PMID: 34773514)
      Circulation. 2021 Mar 16;143(11):1157-1172. (PMID: 33720773)
      Nature. 2015 Aug 20;524(7565):309-314. (PMID: 26266977)
      J Mol Cell Cardiol. 2022 Dec;173:16-24. (PMID: 36084743)
      J Tradit Chin Med. 2022 Oct;42(5):723-731. (PMID: 36083479)
      Biochem Pharmacol. 2022 Mar;197:114891. (PMID: 34968482)
    • Grant Information:
      81960843 Effect of Phosphatase and Tensin Homolog-induced Putative Kinase 1/Parkin Mediated Mitochondrial Autophagy on Myocardial Injury in Chronic Kidney Disease and the Intervention Mechanism of Shenshuai Recipe; No. [2022] 7 Training Plan for Young and Middle-aged Key Talents Project of Traditional Chinese Medicine of Jiangxi Province
    • Contributed Indexing:
      Keywords: PTEN phosphohydrolase; Shenshuai recipe; mitochondrial autophagy; myocardial injury; renal insufficiency, chronic; ubiquitin-protein ligases
    • Accession Number:
      EC 2.3.2.27 (parkin protein)
      0 (Drugs, Chinese Herbal)
      EC 2.3.2.27 (Ubiquitin-Protein Ligases)
      EC 2.7.11.1 (PTEN-induced putative kinase)
      EC 2.7.- (Protein Kinases)
    • Publication Date:
      Date Created: 20241009 Date Completed: 20241009 Latest Revision: 20241016
    • Publication Date:
      20241016
    • Accession Number:
      PMC11462534
    • Accession Number:
      10.19852/j.cnki.jtcm.20231231.001
    • Accession Number:
      39380224