Butin Protects Keratinocytes From Particulate Matter 2.5 and Ultraviolet B-Mediated Damages.

Publisher: Blackwell Country of Publication: England NLM ID: 9013641 Publication Model: Print Cited Medium: Internet ISSN: 1600-0781 (Electronic) Linking ISSN: 09054383 NLM ISO Abbreviation: Photodermatol Photoimmunol Photomed Subsets: MEDLINE

Publication: <2010-> : Oxford : Blackwell
Original Publication: Copenhagen : Munksgaard, c1990-

Ultraviolet Rays*/adverse effects ; Particulate Matter*/adverse effects ; Keratinocytes*/metabolism ; Keratinocytes*/drug effects ; Keratinocytes*/radiation effects ; Keratinocytes*/pathology ; Apoptosis*/drug effects ; Apoptosis*/radiation effects ; DNA Damage*/drug effects; Humans ; HaCaT Cells ; Oxidative Stress/drug effects ; Oxidative Stress/radiation effects ; Reactive Oxygen Species/metabolism ; Lipid Peroxidation/drug effects ; Lipid Peroxidation/radiation effects ; Cell Survival/drug effects ; Cell Line

Background: Butin is a naturally occurring compound with a wide range of medicinal properties, including anti-inflammatory, anti-arthritic, and antioxidant properties. Particulate matter 2.5 (PM 2.5 ) and ultraviolet B (UVB) radiation contribute to skin cell damage via the induction of oxidative stress.
Methods: This study sought to assess the protective effects of butin against damage triggered by PM 2.5 and UVB in human HaCaT keratinocytes. Assessments were performed to evaluate cell viability, apoptosis, and cellular component damage.
Results: Butin exhibited its protective ability via the inhibition of PM 2.5 -induced reactive oxygen species generation, lipid peroxidation, DNA damage, protein carbonylation, and mitochondrial damage. Butin reduced the PM 2.5 -induced c-Fos and phospho-c-Jun protein levels as well as mitogen-activated protein kinase. Furthermore, butin mitigated PM 2.5 - and UVB-induced apoptosis.
Conclusion: Butin had the potential as a pharmaceutical candidate for treating skin damage caused by PM 2.5 and UVB exposure.
(© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

A. B. Omer, M. H. Dalhat, M. K. Khan, et al., “Butin Mitigates Memory Impairment in Streptozotocin‐Induced Diabetic Rats by Inhibiting Oxidative Stress and Inflammatory Responses,” Metabolites 12, no. 11 (2022): 1050.
S. I. Alzarea, A. F. Alasmari, A. S. Alanazi, et al., “Butin Attenuates Arthritis in Complete Freund's Adjuvant‐Treated Arthritic Rats: Possibly Mediated by Its Antioxidant and Anti‐Inflammatory Actions,” Frontiers in Pharmacology 13 (2022): 810052.
F. A. Al‐Abbasi, “Neuroprotective Effect of Butin Against Rotenone‐Induced Parkinson's Disease Mediated by Antioxidant and Anti‐Inflammatory Actions Through Paraoxonase‐1‐Induction,” Journal of Taibah University for Science 6, no. 1 (2022): 944–953.
S. Alshehri, F. A. Al‐Abbasi, M. M. Ghoneim, et al., “Anti‐Huntington's Effect of Butin in 3‐Nitropropionic Acid‐Treated Rats: Possible Mechanism of Action,” Neurotoxicity Research 40, no. 1 (2022): 66–77.
T. Li, R. Hu, Z. Chen, et al., “Fine Particulate Matter (PM2.5): The Culprit for Chronic Lung Diseases in China,” Chronic Diseases and Translational Medicine 4, no. 3 (2018): 176–186.
H. L. Chen, C. P. Li, C. S. Tang, et al., “Risk Assessment for People Exposed to PM2.5 and Constituents at Different Vertical Heights in an Urban Area of Taiwan,” Atmosphere 11, no. 11 (2020): 1145.
I. M. N. Molagoda, M. H. D. Kavinda, Y. H. Choi, et al., “Fisetin Protects HaCaT Human Keratinocytes From Fine Particulate Matter (PM2.5)‐Induced Oxidative Stress and Apoptosis by Inhibiting the Endoplasmic Reticulum Stress Response,” Antioxidants 10, no. 9 (2021): 1492.
R. Li, R. Zhou, and J. Zhang, “Function of PM2.5 in the Pathogenesis of Lung Cancer and Chronic Airway Inflammatory Diseases,” Oncology Letters 15, no. 5 (2018): 7506–7514.
H. M. U. L. Herath, M. J. Piao, K. A. Kang, et al., “Hesperidin Exhibits Protective Effects Against PM2.5‐Mediated Mitochondrial Damage, Cell Cycle Arrest, and Cellular Senescence in Human HaCaT Keratinocytes,” Molecules 27, no. 15 (2022): 4800.
A. X. Zhen, M. J. Piao, Y. J. Hyun, et al., “Purpurogallin Protects Keratinocytes From Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5,” Biomolecules & Therapeutics 27, no. 4 (2019): 395–403.
T. Y. Lin, P. Y. Wu, C. W. Hou, et al., “Protective Effects of Sesamin Against UVB‐Induced Skin Inflammation and Photodamage In Vitro and In Vivo,” Biomolecules 9, no. 9 (2019): 479.
L. Zhang, S. Liang, Z. Zhang, et al., “Protective Effects of ζ‐Carotene‐Like Compounds Against Acute UVB‐Induced Skin Damage,” International Journal of Molecular Sciences 24, no. 18 (2023): 13970.
A. R. Hernández, B. Vallejo, T. Ruzgas, and S. Björklund, “The Effect of UVB Irradiation and Oxidative Stress on the Skin Barrier‐A New Method to Evaluate Sun Protection Factor Based on Electrical Impedance Spectroscopy,” Sensors 19, no. 10 (2019): 2376.
Z. Wen, W. Liu, X. Li, et al., “A Protective Role of the NRF2‐Keap1 Pathway in Maintaining Intestinal Barrier Function,” Oxidative Medicine and Cellular Longevity 2019 (2019): 1759149.
E. C. A. Eleutherio, R. S. Silva Magalhães, B. A. de Araújo, J. R. Monteiro Neto, and P. L. de Holanda, “SOD1, More Than Just an Antioxidant,” Archives of Biochemistry and Biophysics 697 (2021): 108701.
A. X. Zhen, Y. J. Hyun, M. J. Piao, et al., “Eckol Inhibits Particulate Matter 2.5‐Induced Skin Keratinocyte Damage via MAPK Signaling Pathway,” Marine Drugs 17, no. 8 (2019): 444.
Y. J. Hyun, M. J. Piao, K. A. Kang, et al., “Effect of Fermented Fish Oil on Fine Particulate Matter‐Induced Skin Aging,” Marine Drugs 17, no. 1 (2019): 61.
S. Y. Lara Aparicio, Á. D. J. Laureani Fierro, G. E. Aranda Abreu, et al., “Current Opinion on the Use of c‐Fos in Neuroscience,” Neuroscience 3, no. 4 (2022): 687–702.
K. A. Kang, J. H. Lee, S. Chae, et al., “Butin Decreases Oxidative Stress‐Induced 8‐Hydroxy‐2′‐Deoxyguanosine Levels via Activation of Oxoguanine Glycosylase 1,” Chemico‐Biological Interactions 181, no. 3 (2009): 338–342.
R. Zhang, S. Chae, J. H. Lee, and J. W. Hyun, “The Cytoprotective Effect of Butin Against Oxidative Stress Is Mediated by the Up‐Regulation of Manganese Superoxide Dismutase Expression Through a PI3K/Akt/Nrf2‐Dependent Pathway,” Journal of Cellular Biochemistry 113 (2012): 1987–1997.
R. Zhang, K. A. Kang, M. J. Piao, et al., “Butin Reduces Oxidative Stress‐Induced Mitochondrial Dysfunction via Scavenging of Reactive Oxygen Species,” Food and Chemical Toxicology 48 (2010): 922–927.
R. Zhang, S. Chae, K. A. Kang, et al., “Protective Effect of Butin Against Hydrogen Peroxide‐Induced Apoptosis by Scavenging Reactive Oxygen Species and Activating Antioxidant Enzymes,” Molecular and Cellular Biochemistry 318 (2008): 33–42.
R. Zhang, I. K. Lee, M. J. Piao, et al., “Butin (7,3′,4′‐Trihydroxydihydroflavone) Reduces Oxidative Stress‐Induced Cell Death via Inhibition of the Mitochondria‐Dependent Apoptotic Pathway,” International Journal of Molecular Sciences 12 (2011): 3871–3887.
L. Chao, B. Feng, H. Liang, X. Zhao, and J. Song, “Particulate Matter and Inflammatory Skin Diseases: From Epidemiological and Mechanistic Studies,” Science of the Total Environment 905 (2023): 167111.
Y. Wang, D. Li, L. Song, and H. Ding, “Ophiopogonin D Attenuates PM2.5‐Induced Inflammation via Suppressing the AMPK/NF‐κB Pathway in Mouse Pulmonary Epithelial Cells,” Experimental and Therapeutic Medicine 20 (2020): 139.
I. M. Dijkhoff, B. Drasler, B. B. Karakocak, et al., “Impact of Airborne Particulate Matter on Skin: A Systematic Review From Epidemiology to In Vitro Studies,” Particle and Fibre Toxicology 17, no. 1 (2020): 35.
A. Singh, R. Kukreti, L. Saso, and S. Kukreti, “Oxidative Stress: A Key Modulator in Neurodegenerative Diseases,” Molecules 24, no. 8 (2019): 1583.
M. V. Irazabal and V. E. Torres, “Reactive Oxygen Species and Redox Signaling in Chronic Kidney Disease,” Cells 9, no. 6 (2020): 1342.
P. R. Angelova, N. Esteras, and A. Y. Abramov, “Mitochondria and Lipid Peroxidation in the Mechanism of Neurodegeneration: Finding Ways for Prevention,” Medicinal Research Reviews 41, no. 2 (2021): 770–784.
L. J. Su, J. H. Zhang, H. Gomez, et al., “Reactive Oxygen Species‐Induced Lipid Peroxidation in Apoptosis, Autophagy, and Ferroptosis,” Oxidative Medicine and Cellular Longevity 2019 (2019): 5080843.
M. J. Piao, M. J. Ahn, K. A. Kang, et al., “Particulate Matter 2.5 Damages Skin Cells by Inducing Oxidative Stress, Subcellular Organelle Dysfunction, and Apoptosis,” Archives of Toxicology 92, no. 6 (2018): 2077–2091.
B. Honrath, I. Metz, N. Bendridi, J. Rieusset, C. Culmsee, and A. M. Dolga, “Glucose‐Regulated Protein 75 Determines ER‐Mitochondrial Coupling and Sensitivity to Oxidative Stress in Neuronal Cells,” Cell Death Discovery 3 (2017): 17076.
L. Chen, Q. Sun, D. Zhou, et al., “HINT2 Triggers Mitochondrial Ca2+ Influx by Regulating the Mitochondrial Ca2+ Uniporter (MCU) Complex and Enhances Gemcitabine Apoptotic Effect in Pancreatic Cancer,” Cancer Letters 411 (2017): 106–116.
L. Zhao, Q. Gu, L. Xiang, et al., “Curcumin Inhibits Apoptosis by Modulating Bax/Bcl‐2 Expression and Alleviates Oxidative Stress in Testes of Streptozotocin‐Induced Diabetic Rats,” Therapeutics and Clinical Risk Management 13 (2017): 1099–1105.
J. M. Han, H. Y. Song, K. I. Kim, et al., “Polysaccharides From Annona muricata Leaves Protect Against Cisplatin‐Induced Cytotoxicity in Macrophages by Alleviating Mitochondrial Dysfunction,” Molecular Medicine Reports 27, no. 1 (2023): 16.
T. J. Wu, C. Y. Lin, C. H. Tsai, Y. L. Huang, and C. H. Tang, “Glucose Suppresses IL‐1β‐Induced MMP‐1 Expression Through the FAK, MEK, ERK, and AP‐1 Signaling Pathways,” Environmental Toxicology 33 (2018): 1061–1068.
J. Chen, C. Ye, C. Wan, et al., “The Roles of c‐Jun N‐Terminal Kinase (JNK) in Infectious Diseases,” International Journal of Molecular Sciences 22 (2021): 9640.
J. Yue and J. M. López, “Understanding MAPK Signaling Pathways in Apoptosis,” International Journal of Molecular Sciences 21 (2020): 2346.
M. J. Piao, K. A. Kang, Y. S. Ryu, et al., “The Red Algae Compound 3‐Bromo‐4,5‐Dihydroxybenzaldehyde Protects Human Keratinocytes on Oxidative Stress‐Related Molecules and Pathways Activated by UVB Irradiation,” Marine Drugs 15, no. 9 (2017): 268.
K. Mokrzyński, O. Krzysztyńska‐Kuleta, M. Zawrotniak, M. Sarna, and T. Sarna, “Fine Particulate Matter‐Induced Oxidative Stress Mediated by UVA‐Visible Light Leads to Keratinocyte Damage,” International Journal of Molecular Sciences 22, no. 19 (2021): 10645.
L. Guerrero‐Navarro, P. Jansen‐Durr, and M. Cavinato, “Synergistic Interplay of UV Radiation and Urban Particulate Matter Induces Impairment of Autophagy and Alters Cellular Fate in Senescence‐Prone Human Dermal Fibroblasts,” Aging Cell 23, no. 4 (2024): e14086.
W. J. Sim, E. Lee, S. Yun, W. Lim, and T. G. Lim, “Particulate Matter‐Induced Skin Inflammation Is Suppressed by Polyphenol‐Enriched Dietary Supplement via Inhibition of the AhR/ARNT Signaling Pathway,” Journal of Functional Foods 106 (2023): 105593.

Jeju National University Hospital; RS-2023-00270936 Ministry of Education

Keywords: UVB; butin; particulate matter

0 (Particulate Matter)
0 (Reactive Oxygen Species)

Date Created: 20241005 Date Completed: 20241005 Latest Revision: 20241005

20241006

10.1111/phpp.13001

39368082