Genome-wide association study of cassava brown streak disease resistance in cassava germplasm conserved in South America.

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  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • Subject Terms:
      Manihot*/genetics ; Manihot*/virology ; Manihot*/parasitology ; Genome-Wide Association Study* ; Disease Resistance*/genetics ; Plant Diseases*/genetics ; Plant Diseases*/virology ; Polymorphism, Single Nucleotide*; South America ; Genotype ; Genome, Plant ; Potyviridae
    • Abstract:
      Cassava (Manihot esculenta Crantz) is a vital carbohydrate source for over 800 million people globally, yet its production in East Africa is severely affected by cassava brown streak disease (CBSD). Genebanks, through ex-situ conservation, play a pivotal role in preserving crop diversity, providing crucial resources for breeding resilient and disease-resistant crops. This study genotyped 234 South American cassava accessions conserved at the CIAT genebank, previously phenotyped for CBSD resistance by an independent group, to perform a genome-wide association analysis (GWAS) to identify genetic variants associated with CBSD resistance. Our GWAS identified 35 single nucleotide polymorphism (SNP) markers distributed across various chromosomes, associated with disease severity or the presence/absence of viral infection. Markers were annotated within or near genes previously identified with functions related to pathogen recognition and immune response activation. Using the SNP candidates, we screened the world's largest cassava collection for accessions with a higher frequency of favorable genotypes, proposing 35 accessions with potential resistance to CBSD. Our results provide insights into the genetics of CBSD resistance and highlight the importance of genetic resources to equip breeders with the raw materials needed to develop new crop varieties resistant to pests and diseases.
      (© 2024. The Author(s).)
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    • Contributed Indexing:
      Keywords: Cassava brown streak disease (CBSD); Disease resistance; Ex-situ conservation; Genetic resources; Genome-wide association study (GWAS); Molecular breeding.
    • Subject Terms:
      Cassava brown streak virus
    • Publication Date:
      Date Created: 20241004 Date Completed: 20241004 Latest Revision: 20241007
    • Publication Date:
      20241007
    • Accession Number:
      PMC11452518
    • Accession Number:
      10.1038/s41598-024-74161-6
    • Accession Number:
      39367150