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Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards.
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- Additional Information
- Source:
Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
- Publication Information:
Original Publication: [London] : Nature Pub. Group
- Subject Terms:
- Abstract:
In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as a set of candidate plasma reference materials (RM 8231). Participants either utilized a provided validated method and/or their method of choice. Mean concentration values, and intra- and inter-laboratory coefficients of variation (CV) were calculated using single-point and multi-point calibrations, respectively. These results are the most precise (intra-laboratory CVs ≤ 4.2%) and concordant (inter-laboratory CVs < 14%) community-derived absolute concentration values reported to date for four clinically used ceramides in the commonly analyzed SRM 1950. We demonstrate that calibration using authentic labelled standards dramatically reduces data variability. Furthermore, we show how the use of shared RM can correct systematic quantitative biases and help in harmonizing lipidomics. Collectively, the results from the present study provide a significant knowledge base for translation of lipidomic technologies to future clinical applications that might require the determination of reference intervals (RIs) in various human populations or might need to estimate reference change values (RCV), when analytical variability is a key factor for recall during multiple testing of individuals.
(© 2024. The Author(s).)
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- Grant Information:
PG/2019/34923 United Kingdom BHF_ British Heart Foundation
- Accession Number:
0 (Ceramides)
- Publication Date:
Date Created: 20241003 Date Completed: 20241003 Latest Revision: 20241204
- Publication Date:
20241204
- Accession Number:
PMC11449902
- Accession Number:
10.1038/s41467-024-52087-x
- Accession Number:
39362843
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