Selenomethionine alleviates Aeromonas hydrophila-induced oxidative stress and ferroptosis via the Nrf2/HO1/GPX4 pathway in grass carp.

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  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE
    • Publication Information:
      Original Publication: London ; New York : Academic Press, c1991-
    • Subject Terms:
      Carps*/immunology ; Aeromonas hydrophila*/physiology ; Ferroptosis*/drug effects ; Fish Diseases*/immunology ; Oxidative Stress*/drug effects ; Gram-Negative Bacterial Infections*/veterinary ; Gram-Negative Bacterial Infections*/immunology ; Selenomethionine*/pharmacology ; Selenomethionine*/administration & dosage ; Fish Proteins*/metabolism ; NF-E2-Related Factor 2*/metabolism ; NF-E2-Related Factor 2*/genetics; Animals ; Phospholipid Hydroperoxide Glutathione Peroxidase/metabolism ; Heme Oxygenase-1/metabolism ; Heme Oxygenase-1/genetics ; Signal Transduction/drug effects
    • Abstract:
      Aeromonas hydrophila infection is a severe, acute, and life-threatening disease affecting grass carp (Ctenopharyngodon idella) in aquaculture. Ferroptosis is a novel form of cell death characterized by the accumulation of free iron and harmful lipid peroxides within cells. While selenomethionine (Se-Met) is known to effectively inhibit ferroptosis and alleviate cell damage, its ability to counteract oxidative stress and ferroptosis induced by A. hydrophila remains unclear. The objective of this study is to reveal the possible mechanism behind the ferroptosis phenomenon during A. hydrophila infection. We established a macrophage model of A. hydrophila invasion to monitor the dynamic changes in iron metabolism markers to evaluate the correlation between ferroptotic stress and A. hydrophila infection. A. hydrophila infection induces cytotoxicity and mitochondrial membrane damage via ferroptosis. This damage is attributed to the accumulation of lipid peroxides due to intracellular ferrous ion overload and glutathione depletion. Supplementation of Se-Met reduced mitochondrial damage, enhanced antioxidant enzyme activity and reduced ferroptosis by activating the Nrf2/HO1/GPX4 axis. These findings provide new insights into the regulatory mechanisms of A. hydrophila-induced ferroptosis in teleosts and suggest that targeted inhibition of ferroptosis may offer a novel therapeutic strategy for managing A. hydrophila infections.
      (Copyright © 2024 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: Aeromonas hydrophila; Ferroptosis; Grass carp; Nrf2/HO1/GPX4; Oxidative stress; Selenomethionine
    • Accession Number:
      964MRK2PEL (Selenomethionine)
      0 (Fish Proteins)
      0 (NF-E2-Related Factor 2)
      EC 1.11.1.12 (Phospholipid Hydroperoxide Glutathione Peroxidase)
      EC 1.14.14.18 (Heme Oxygenase-1)
    • Publication Date:
      Date Created: 20240930 Date Completed: 20241113 Latest Revision: 20241113
    • Publication Date:
      20241114
    • Accession Number:
      10.1016/j.fsi.2024.109927
    • Accession Number:
      39349229