Multilayer Gelatin-Supported BMP-9 Coating Promotes Osteointegration and Neo-Bone Formation at the n-CDHA/PAA Composite Biomaterial-Bone Interface.

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  • Additional Information
    • Source:
      Publisher: IMR Press Country of Publication: Singapore NLM ID: 101612996 Publication Model: Print Cited Medium: Internet ISSN: 2768-6698 (Electronic) Linking ISSN: 27686698 NLM ISO Abbreviation: Front Biosci (Landmark Ed) Subsets: MEDLINE
    • Publication Information:
      Publication: 2022- : Singapore : IMR Press
      Original Publication: Searington, NY : Frontiers in Bioscience
    • Subject Terms:
      Growth Differentiation Factor 2*/metabolism ; Gelatin*/chemistry ; Osseointegration*/drug effects ; Durapatite*/chemistry ; Durapatite*/pharmacology ; Coated Materials, Biocompatible*/chemistry ; Mesenchymal Stem Cells*/drug effects ; Mesenchymal Stem Cells*/metabolism ; Osteogenesis*/drug effects; Animals ; Rabbits ; Humans ; Bone Regeneration/drug effects ; Femoral Fractures/surgery ; Tissue Engineering/methods ; Biocompatible Materials/chemistry
    • Abstract:
      Background: The development of biomaterials capable of accelerating bone wound repair is a critical focus in bone tissue engineering. This study aims to evaluate the osteointegration and bone regeneration potential of a novel multilayer gelatin-supported Bone Morphogenetic Protein 9 (BMP-9) coated nano-calcium-deficient hydroxyapatite/poly-amino acid (n-CDHA/PAA) composite biomaterials, focusing on the material-bone interface, and putting forward a new direction for the research on the interface between the coating material and bone.
      Methods: The BMP-9 recombinant adenovirus (Adenovirus (Ad)-BMP-9/Bone Marrow Mesenchymal Stem Cells (BMSc)) was produced by transfecting BMSc and supported using gelatin (Ad-BMP-9/BMSc/Gelatin (GT). Multilayer Ad-BMP-9/BMSc/GT coated nano-calcium deficient hydroxyapatite/polyamino acid (n-CDHA/PAA) composite biomaterials were then prepared and co-cultured with MG63 cells for 10 days, with biocompatibility assessed through microscopy, Cell Counting Kit-8 (CCK-8), and alkaline phosphatase (ALP) assays. Subsequently, multilayer Ad-BMP-9/BMSc/GT coated n-CDHA/PAA composite biomaterial screws were fabricated, and the adhesion of the coating to the substrate was observed using scanning electron microscopy (SEM). In vivo studies were conducted using a New Zealand White rabbit intercondylar femoral fracture model. The experimental group was fixed with screws featuring multilayer Ad-BMP-9/BMSc/GT coatings, while the control groups used medical metal screws and n-CDHA/PAA composite biomaterial screws. Fracture healing was monitored at 1, 4, 12, and 24 weeks, respectively, using X-ray observation, Micro-CT imaging, and SEM. Integration at the material-bone interface and the condition of neo-tissue were assessed through these imaging techniques.
      Results: The Ad-BMP-9/GT coating significantly enhanced MG63 cell adhesion, proliferation, and differentiation, while increasing BMP-9 expression in vitro . In vivo studies using a rabbit femoral fracture model confirmed the biocompatibility and osteointegration potential of the multilayer Ad-BMP-9/BMSc/GT coated n-CDHA/PAA composite biomaterial screws. Compared to control groups (medical metal screws and n-CDHA/PAA composite biomaterial screws), this material demonstrated faster fracture healing, stronger osteointegration, and facilitated new bone tissue formation with increased calcium deposition at the material-bone interface.
      Conclusion: The multilayer GT-supported BMP-9 coated n-CDHA/PAA composite biomaterials have demonstrated favorable osteogenic cell interface performance, both in vitro and in vivo . This study provides a foundation for developing innovative bone repair materials, holding promise for significant advancements in clinical applications.
      (© 2024 The Author(s). Published by IMR Press.)
    • Grant Information:
      cstc2021jcyj-msxmX0832 General Project of Chongqing Municipal Natural Science Foundation; CSTB2022BSXM-JCX0072 Doctor Through Train Project of Chongqing Science and Technology Commission
    • Contributed Indexing:
      Keywords: bone tissue engineering; composite biomaterial; gelatin-supported BMP-9 coating; n-CDHA/PAA
    • Accession Number:
      0 (Growth Differentiation Factor 2)
      9000-70-8 (Gelatin)
      91D9GV0Z28 (Durapatite)
      0 (Coated Materials, Biocompatible)
      0 (Biocompatible Materials)
    • Publication Date:
      Date Created: 20240930 Date Completed: 20240930 Latest Revision: 20240930
    • Publication Date:
      20240930
    • Accession Number:
      10.31083/j.fbl2909326
    • Accession Number:
      39344336