Novel genomic and phenotypic traits of polyhydroxyalkanoate-producing bacterium ZZQ-149, the type strain of Halomonas qinghailakensis.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, [2001-
    • Subject Terms:
      Polyhydroxyalkanoates*/metabolism ; Halomonas*/genetics ; Halomonas*/metabolism ; Halomonas*/classification ; Genome, Bacterial*/genetics ; Phylogeny* ; RNA, Ribosomal, 16S*/genetics; China ; Phenotype ; Genomics/methods ; Geologic Sediments/microbiology ; DNA, Bacterial/genetics ; Lakes/microbiology ; Sequence Analysis, DNA
    • Abstract:
      Background: Polyhydroxyalkanoates (PHAs) are optimal potential materials for industrial and medical uses, characterized by exceptional sustainability, biodegradability, and biocompatibility. These are primarily from various bacteria and archaea. Bacterial strains with effective PHA formation capabilities and minimal production cost form the foundation for PHA production. Detailed genomic analysis of these PHA-generating bacteria is vital to understand their PHA production pathways and enhance their synthesis capability.
      Results: ZZQ-149, a halophilic, PHA-producing bacterium, was isolated from the sediment of China's Qinghai Lake. Here, we decoded the full genome of ZZQ-149 using Single Molecule Real Time (SMRT) technology based on PacBio RS II platform, coupled with Illumina sequencing platforms. Physiological, chemotaxonomic traits, and phylogenetic analysis based on 16 S rRNA gene and single copy core genes of ninety-nine Halomonas type strains identified ZZQ-149 as the type strain of Halomonas qinghailakensis. Furthermore, a low average nucleotide identity (ANI, < 95%) delineated the genetic differences between ZZQ-149 and other Halomonas species. The ZZQ-149 genome, with a DNA G + C content of 52%, comprises a chromosome (3, 798, 069 bps) and a plasmid (6, 107 bps). The latter encodes the toxin-antitoxin system, BrnT/BrnA. Through comprehensive genome sequencing and analysis, we identified multiple PHA-synthesizing enzymes and an unprecedented combination of eight PHA-synthesizing pathways in ZZQ-149.
      Conclusions: Being a halophilic, PHA-producing bacterium, ZZQ-149 exhibits potential as a high PHA producer for engineered bacteria via genome editing while ensuring low-cost PHA production through continuous, unsterilized fermentation.
      (© 2024. The Author(s).)
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    • Grant Information:
      2021RZ038 Research Funding of Wuhan Polytechnic University; 2021Y10 Research and Innovation Initiatives of Wuhan Polytechnic University; 2022ZTSJJ7 Open Fund from Key Laboratory of Integrated Pests Management on Crops in Central China/Hubei Key Laboratory of Crop Diseases, Insect Pests and Weeds Control; D20231606 Science and Technology Research Project of Hubei Education Department
    • Contributed Indexing:
      Keywords: Genome; Halomonas qinghailakensis; Halophile; Pathway; Polyhydroxyalkanoate
    • Accession Number:
      0 (Polyhydroxyalkanoates)
      0 (RNA, Ribosomal, 16S)
      0 (DNA, Bacterial)
    • Publication Date:
      Date Created: 20240928 Date Completed: 20240929 Latest Revision: 20241001
    • Publication Date:
      20241001
    • Accession Number:
      PMC11438105
    • Accession Number:
      10.1186/s12866-024-03532-w
    • Accession Number:
      39342120