Blood Coagulation Activities and Influence on DNA Condition of Alginate-Calcium Composites Prepared by Freeze-Drying Technique.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101213729 Publication Model: Electronic Cited Medium: Internet ISSN: 1660-3397 (Electronic) Linking ISSN: 16603397 NLM ISO Abbreviation: Mar Drugs Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, [2003]-
    • Subject Terms:
      Alginates*/chemistry ; Freeze Drying* ; Blood Coagulation*/drug effects ; DNA*/chemistry ; Calcium*/chemistry; Calcium Chloride/chemistry ; Partial Thromboplastin Time ; Animals ; Porosity ; Humans ; Cross-Linking Reagents/chemistry
    • Abstract:
      The aim of this research was to synthesize and characterize alginate-calcium composites using a freeze-drying method, with a focus on their potential applications in biomedicine. This study specifically explored the biochemical properties of these composites, emphasizing their role in blood coagulation and their capacity to interact with DNA. Additionally, the research aimed to assess how the cross-linking process influences the structural and chemical characteristics of the composites. Detailed analyses, including microscopic examination, surface area assessment, and atomic absorption spectrometry, yielded significant results. The objective of this study was to examine the impact of calcium chloride concentration on the calcium content in alginate composites. Specifically, the study assessed how varying concentrations of the cross-linking solution (ranging from 0.5% to 2%) influence the calcium ion saturation within the composites. This investigation is essential for understanding the physicochemical properties of the materials, including calcium content, porosity, and specific surface area. The results are intended to identify the optimal cross-linking conditions that maximize calcium enrichment efficiency while preserving the material's structural integrity. The study found that higher calcium chloride concentrations in alginate cross-linking improve the formation of a porous structure, enhanced by two-stage freeze-drying. Increased calcium levels led to a larger surface area and pore volume, and significantly higher calcium content. Furthermore, assays of activated partial thromboplastin time (aPTT) showed a reduction in clotting time for alginate composites containing calcium ions, indicating their potential as hemostatic agents. The aPTT test showed shorter clotting times with higher calcium ion concentrations, without enhanced activation of the extrinsic clotting pathway. The developed alginate material with calcium effectively supports hemostasis and reduces the risk of infection. The study also explored the capacity of these composites to interact with and modify the structure of plasmid DNA, underscoring their potential for future biomedical applications.
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    • Grant Information:
      2022/04/Y/ST4/00157 National Centre for Research and Development
    • Contributed Indexing:
      Keywords: activated partial thromboplastin time (aPTT); alginate; blood coagulation; calcium; composite; plasmid relaxation assay; polymer functionalization; prothrombin time (PT)
    • Accession Number:
      0 (Alginates)
      9007-49-2 (DNA)
      SY7Q814VUP (Calcium)
      M4I0D6VV5M (Calcium Chloride)
      0 (Cross-Linking Reagents)
    • Publication Date:
      Date Created: 20240927 Date Completed: 20240927 Latest Revision: 20240929
    • Publication Date:
      20240929
    • Accession Number:
      PMC11433402
    • Accession Number:
      10.3390/md22090415
    • Accession Number:
      39330295