Moment analysis method for the determination of permeation kinetics of coumarin at lipid bilayers of liposomes by using capillary electrophoresis.

Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 8204476 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-2683 (Electronic) Linking ISSN: 01730835 NLM ISO Abbreviation: Electrophoresis Subsets: MEDLINE

Publication: : Weinheim : Wiley-VCH
Original Publication: [Weinheim, Germany] : Verlag Chemie, [1980-

Liposomes*/chemistry ; Coumarins*/chemistry ; Lipid Bilayers*/chemistry ; Electrophoresis, Capillary*/methods; Kinetics ; Adsorption ; Permeability ; Phospholipids/chemistry

A method was developed for studying mass transfer kinetics at lipid bilayers of liposomes. Elution peaks of coumarin were measured by liposome electrokinetic chromatography (LEKC). Four types of phospholipids having different alkyl chains were used for preparing liposomes, which were used as pseudo-stationary phases in LEKC systems. Rate constants of permeation across lipid bilayers of liposomes or of adsorption at lipid membranes were determined by analyzing the first absolute and second central moments of the elution peaks measured by LEKC. The rate constants of permeation or adsorption tend to decrease with an increase in the carbon number of the alkyl chains of phospholipids. It was demonstrated that the moment analysis of elution peak profiles measured by LEKC is effective for determining lipid membrane permeability or adsorption kinetics. Compared with other conventional techniques, the method has some advantages for studying mass transfer kinetics at lipid bilayers. Solute permeation across or solute adsorption at real lipid bilayers of liposomes is analyzed. The principle of the method is the analysis of separation behavior in LEKC, which is different from that of the other ones. It is expected that the method contributes to the kinetic study of mass transfer at lipid bilayers from various perspectives.
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23K04795 JSPS KAKENHI

Keywords: adsorption rate constant; lipid membrane permeability; liposome electrokinetic chromatography; mass transfer kinetics; moment analysis theory

0 (Liposomes)
0 (Coumarins)
0 (Lipid Bilayers)
A4VZ22K1WT (coumarin)
0 (Phospholipids)

Date Created: 20240927 Date Completed: 20241221 Latest Revision: 20241221

20241222

10.1002/elps.202400100

39329504