Expanding the field of view - a simple approach for interactive visualisation of electron microscopy data.

Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE

Publication: Cambridge : Company of Biologists
Original Publication: London.

Microscopy, Electron*/methods; Image Processing, Computer-Assisted/methods ; Humans

The unparalleled resolving power of electron microscopy is both a blessing and a curse. At 30,000× magnification, 1 µm corresponds to 3 cm in the image and the field of view is only a few micrometres or less, resulting in an inevitable reduction in the spatial data available in an image. Consequently, the gain in resolution is at the cost of loss of the contextual 'reference space', which is crucial for understanding the embedded structures of interest. This problem is particularly pronounced in immunoelectron microscopy, where the detection of a gold particle is crucial for the localisation of specific molecules. The common solution of presenting high-magnification and overview images side by side often insufficiently represents the cellular environment. To address these limitations, we propose here an interactive visualization strategy inspired by digital maps and GPS modules which enables seamless transitions between different magnifications by dynamically linking virtual low magnification overview images with primary high-resolution data. By enabling dynamic browsing, it offers the potential for a deeper understanding of cellular landscapes leading to more comprehensive analysis of the primary ultrastructural data.
Competing Interests: Competing interests The author declares no competing or financial interests.
(© 2024. Published by The Company of Biologists Ltd.)

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Universitetet i Oslo

Keywords: Data communication; Electron microscopy; Interactive visualization; Reference space; Teaching

Date Created: 20240926 Date Completed: 20241023 Latest Revision: 20241103

20241103

PMC11529876

10.1242/jcs.262198

39324375