DNA Framework-Templated Synthesis of Copper Cluster Nanozyme with Enhanced Activity and Specificity.

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  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, D.C. : American Chemical Society
    • Subject Terms:
      Copper*/chemistry ; DNA*/chemistry ; Biosensing Techniques*/methods ; Hydrogen Peroxide*/chemistry; Humans ; Catalysis ; Nanostructures/chemistry ; Metal Nanoparticles/chemistry ; Limit of Detection
    • Abstract:
      Nanozymes have been developed to overcome the inherent limitations of natural enzymes, such as their low stability and high cost. However, their efficacy has been hindered by their relatively low specificity and activity. Here, we demonstrate the self-assembly of individual copper nanoclusters (CuNCs) via a simple yet fast (10 min) DNA nanosheet (DNS)-templated method, enhancing the peroxidase-like activity and specificity of CuNCs. Furthermore, we demonstrate the successful assembly of CuNCs on different DNA nanostructures by atomic force microscopy (AFM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). The resulting micron-scale ultrathin DNA nanosheet-templated CuNCs (DNS@CuNCs) exhibit exceptional catalytic activity, with a specific activity reaching 1.79 × 10 3 U mg -1 . Investigation into the catalytic process reveals that the enhanced activity and specificity arise from disparities in active intermediate content before and after CuNCs assembly. Significantly, the DNS@CuNCs-based biosensor demonstrates remarkable anti-interference capabilities, enabling the detection of H 2 O 2 in undiluted human serum for the first time with a detection limit of 0.99 μM.
    • Contributed Indexing:
      Keywords: Copper Clusters; DNA Framework; DNA Nanostructure; Nanozyme; Self-Assembly
    • Accession Number:
      789U1901C5 (Copper)
      9007-49-2 (DNA)
      BBX060AN9V (Hydrogen Peroxide)
    • Publication Date:
      Date Created: 20240925 Date Completed: 20241010 Latest Revision: 20241010
    • Publication Date:
      20250114
    • Accession Number:
      10.1021/acsami.4c09208
    • Accession Number:
      39322981