GC-MS metabolomics of French lettuce (Lactuca Sativa L. var capitata) leaves exposed to bisphenol A via the hydroponic media.

Publisher: Springer Country of Publication: United States NLM ID: 101274889 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-3890 (Electronic) Linking ISSN: 15733882 NLM ISO Abbreviation: Metabolomics Subsets: MEDLINE

Original Publication: New York : Springer, c2006-

Benzhydryl Compounds*/analysis ; Lactuca*/metabolism ; Lactuca*/drug effects ; Lactuca*/growth & development ; Lactuca*/chemistry ; Gas Chromatography-Mass Spectrometry*/methods ; Plant Leaves*/metabolism ; Plant Leaves*/drug effects ; Phenols*/metabolism ; Phenols*/analysis ; Metabolomics*/methods ; Hydroponics*/methods; Metabolome/drug effects

Introduction: Bisphenol A (BPA), an organic compound used to produce polycarbonate plastics and epoxy resins, has become a ubiquitous contaminant due to its high-volume production and constant release to the environment. Plant metabolomics can trace the stress effects induced by environmental contaminants to the variation of specific metabolites, making it an alternative way to study pollutants toxicity to plants. Nevertheless, there is an important knowledge gap in metabolomics applications in this area.
Objective: Evaluate the influence of BPA in French lettuce (Lactuca Sativa L. var capitata) leaves metabolic profile by gas chromatography coupled to mass spectrometry (GC-MS) using a hydroponic system.
Methods: Lettuces were cultivated in the laboratory to minimize biological variation and were analyzed 55 days after sowing (considered the plant's adult stage). Hexanoic and methanolic extracts with and without derivatization were prepared for each sample and analyzed by GC-MS.
Results: The highest number of metabolites was obtained from the hexanoic extract, followed by the derivatized methanolic extract. Although no physical differences were observed between control and contaminated lettuce leaves, the multivariate analysis determined a statistically significant difference between their metabolic profiles. Pathway analysis of the most affected metabolites showed that galactose metabolism, starch and fructose metabolism and steroid biosynthesis were significantly affected by BPA exposure.
Conclusions: The preparation of different extracts from the same sample permitted the determination of metabolites with different physicochemical properties. BPA alters the leaves energy and membrane metabolism, plant growth could be affected at higher concentrations and exposition times.
(© 2024. The Author(s).)

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663570 Consejo Nacional de Humanidades Ciencia y Tecnología; IN 212824 Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México; 5000/9026 Programa de Apoyo a la Investigación y el Posgrado

Keywords: Bisphenol A; Chemometrics; Gas chromatography–mass spectrometry; Lettuce; Metabolomics

0 (Benzhydryl Compounds)
MLT3645I99 (bisphenol A)
0 (Phenols)

Date Created: 20240921 Date Completed: 20240921 Latest Revision: 20240924

20240924

PMC11416399

10.1007/s11306-024-02168-1

39306645