Protocol to monitor live-cell, real-time, mitochondrial respiration in mouse muscle cells using the Resipher platform.

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  • Author(s): Triolo M;Triolo M; Khacho M; Khacho M
  • Source:
    STAR protocols [STAR Protoc] 2024 Dec 20; Vol. 5 (4), pp. 103330. Date of Electronic Publication: 2024 Sep 20.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Cambridge, MA] : Cell Press, [2020]-
    • Subject Terms:
      Cell Respiration*/physiology; Animals ; Mice ; Mitochondria/metabolism ; Oxygen Consumption/physiology ; Cell Line ; Myoblasts/metabolism ; Myoblasts/cytology ; Muscle, Skeletal/cytology ; Muscle, Skeletal/metabolism ; Mitochondria, Muscle/metabolism
    • Abstract:
      Mitochondrial function is typically assessed by measuring oxygen consumption at a given time point. However, this approach cannot monitor respiratory changes that occur over time. Here, we present a protocol to measure mitochondrial respiration in freshly isolated muscle stem cells, primary skeletal muscle, and immortalized C2C12 myoblasts in real time using the Resipher platform. We describe steps for preparing and plating cells, performing media changes, setting up the software and device, and analyzing data. This method can be adapted to other cell types. For complete details on the use and execution of this protocol, please refer to Triolo et al. 1 .
      Competing Interests: Declaration of interests The authors declare no competing interests.
      (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Cell Biology; Metabolism; Stem Cells
    • Publication Date:
      Date Created: 20240921 Date Completed: 20241224 Latest Revision: 20241224
    • Publication Date:
      20241224
    • Accession Number:
      PMC11459069
    • Accession Number:
      10.1016/j.xpro.2024.103330
    • Accession Number:
      39305486