Mononuclear cell-mediated modulation of synovial cell metabolism. II. Increased hyaluronic acid synthesis by a monocyte cell factor (MCF).

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  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: United States NLM ID: 0373226 Publication Model: Print Cited Medium: Print ISSN: 0014-4827 (Print) Linking ISSN: 00144827 NLM ISO Abbreviation: Exp Cell Res Subsets: MEDLINE
    • Publication Information:
      Publication: Orlando Fl : Academic Press
      Original Publication: New York, Academic Press.
    • Subject Terms:
      Glycosyltransferases* ; Membrane Proteins* ; Transferases* ; Xenopus Proteins*; Hyaluronic Acid/*biosynthesis ; Proteins/*pharmacology ; Synovial Membrane/*metabolism; Aged ; Cells, Cultured ; Dactinomycin/pharmacology ; Female ; Glucosamine/metabolism ; Glucuronosyltransferase/metabolism ; Humans ; Hyaluronan Synthases ; Indomethacin/pharmacology ; Male ; Middle Aged ; Molecular Weight ; Monokines ; Synovial Membrane/drug effects ; Synovial Membrane/enzymology
    • Abstract:
      Treatment of cultured human synovial cells with a mononuclear cell factor (MCF) enhanced their ability to synthesize glycosaminoglycans (GAG), but GAG repartition between extracellular, pericellular and intracellular compartments was found to be the same as in control. Hyaluronic acid (HA) production, which represents 80-90% of all secreted GAG, was stimulated 2 1/2-3-fold, but the HA molecular weight was not modified. The MCF increased the hyaluronate synthetase activity of synovial cells in similar proportions. Actinomycin D inhibited the increase in hyaluronate synthetase activity produced by MCF, indicating that this increase involves new synthesis of mRNA. Stimulation of both HA synthesis and hyaluronate synthetase activity by MCF was suppressed by 10(-4)-10(-5) M indomethacin (an inhibitor of cyclo-oxygenase), suggesting that MCF effect is prostaglandin-dependent.
    • Accession Number:
      0 (Membrane Proteins)
      0 (Monokines)
      0 (Proteins)
      0 (Xenopus Proteins)
      1CC1JFE158 (Dactinomycin)
      9004-61-9 (Hyaluronic Acid)
      EC 2.- (Transferases)
      EC 2.4.- (Glycosyltransferases)
      EC 2.4.1.17 (Glucuronosyltransferase)
      EC 2.4.1.212 (HAS1 protein, Xenopus)
      EC 2.4.1.212 (Hyaluronan Synthases)
      N08U5BOQ1K (Glucosamine)
      XXE1CET956 (Indomethacin)
    • Publication Date:
      Date Created: 19850901 Date Completed: 19851105 Latest Revision: 20190707
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/0014-4827(85)90231-9
    • Accession Number:
      3930273