Synthesis of colicin Ia neoglycoproteins: tools towards glyco-engineering of bacterial cell surfaces.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101581657 Publication Model: eCollection Cited Medium: Internet ISSN: 2046-2069 (Electronic) Linking ISSN: 20462069 NLM ISO Abbreviation: RSC Adv Subsets: PubMed not MEDLINE
    • Publication Information:
      Original Publication: Cambridge [England] : Royal Society of Chemistry, [2011]-
    • Abstract:
      Colicins are antimicrobial proteins produced by certain strains of Escherichia coli that function as offensive weapons against closely-related competitor strains. Their bactericidal properties and narrow bacterial targeting range has made them of therapeutic interest. Furthermore, the applications of engineered non-bactericidal colicins are of interest as a cell surface-directed protein anchor for decorating E. coli with biomolecules. We previously demonstrated that an engineered non-bacteriocidal colicin E9 could be used to label bacterial cells with multiple biomolecules including glycans. Herein we extend our approach to colicin Ia, constructing mannose-presenting colicin la neoglycoproteins, through N-terminal organocatalyst-mediated protein aldol ligation (OPAL), or maleimide ligation targeting an internal cysteine. This work further highlights the potential utility of engineered colicins for non-genetic glyco-engineering of the E. coli cell surface.
      Competing Interests: There are no conflicts to declare.
      (This journal is © The Royal Society of Chemistry.)
    • References:
      J Biol Chem. 1972 Oct 25;247(20):6524-9. (PMID: 4561939)
      Synth Biol (Oxf). 2018 Jun 06;3(1):ysy004. (PMID: 32995513)
      Biochim Biophys Acta. 2014 Aug;1843(8):1717-31. (PMID: 24746518)
      RSC Adv. 2024 Jan 24;14(6):3723-3729. (PMID: 38268544)
      J Am Soc Mass Spectrom. 2018 Jan;29(1):140-149. (PMID: 29027149)
      Nat Commun. 2016 Mar 07;7:10738. (PMID: 26948702)
      Nat Commun. 2018 Mar 14;9(1):1082. (PMID: 29540681)
      Emerg Top Life Sci. 2017 Apr 21;1(1):65-74. (PMID: 33525816)
      JACS Au. 2024 Jun 12;4(6):2122-2129. (PMID: 38938796)
      Q Rev Biophys. 2012 Feb;45(1):57-103. (PMID: 22085441)
      Nature. 1997 Jan 30;385(6615):461-4. (PMID: 9009197)
      AIChE J. 2021 Dec;67(12):. (PMID: 36329688)
      Microbiol Mol Biol Rev. 2007 Mar;71(1):158-229. (PMID: 17347522)
      EMBO J. 2007 May 16;26(10):2594-604. (PMID: 17464289)
      Chembiochem. 2012 Sep 3;13(13):1874-9. (PMID: 22893609)
      Biosens Bioelectron. 2014 Sep 15;59:404-11. (PMID: 24768820)
      Chemistry. 2024 Jan 2;30(1):e202300145. (PMID: 37814903)
      Nature. 2015 Jul 16;523(7560):333-6. (PMID: 26061769)
      Anal Chem. 2011 Sep 1;83(17):6868-74. (PMID: 21744800)
      Chem Sci. 2018 May 31;9(25):5585-5593. (PMID: 30061990)
    • Publication Date:
      Date Created: 20240916 Latest Revision: 20240918
    • Publication Date:
      20240918
    • Accession Number:
      PMC11394469
    • Accession Number:
      10.1039/d4ra04774e
    • Accession Number:
      39282067