Identification of Cellular Isoschaftoside-Mediated Anti-Senescence Mechanism in RAC2 and LINC00294 .

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Cellular Senescence*/drug effects ; Reactive Oxygen Species*/metabolism ; rac GTP-Binding Proteins*/metabolism ; rac GTP-Binding Proteins*/genetics ; RAC2 GTP-Binding Protein* ; Antioxidants*/pharmacology ; Antioxidants*/chemistry; Humans ; Mitochondria/metabolism ; Mitochondria/drug effects ; Glycosides/pharmacology ; Glycosides/chemistry ; Flavonoids/pharmacology ; Flavonoids/chemistry ; Cell Line

As cellular senescence, reactive oxygen species (ROS) accumulate excessively, causing cellular damage. Flavonoids derived from natural products are known for their antioxidant effects and their ability to delay cellular senescence. Previous studies have attempted to mitigate cellular senescence using flavonoids from natural sources. However, the detailed mechanisms and regulatory targets of some flavonoids exhibiting antioxidant effects have not been fully elucidated. Therefore, we screened a library of flavonoids for antioxidant properties. Isoschaftoside, a glycosidic flavonoid, significantly reduced ROS levels in senescent cells. It was found that mitochondrial function was restored, and dependence on glycolysis was reduced in senescent cells treated with isoschaftoside. Additionally, we identified that isoschaftoside suppresses ROS by reducing the expression of RAC2 and LINC00294 in senescent cells. Taken together, this study establishes a novel mechanism for ROS inhibition and the regulation of cellular senescence by isoschaftoside. Our findings contribute important insights to antioxidant and anti-senescence research.

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HP23C0024 Ministry of Health & Welfare; 2020R1A6A1A0304195411 National Research Foundation of Korea; NRF2021R1A2C1093814 National Research Foundation of Korea; 2019R1A6A1A03031807 National Research Foundation of Korea

Keywords: flavonoid; isoschaftoside; reactive oxygen species (ROS); senescence amelioration

0 (Reactive Oxygen Species)
EC 3.6.5.2 (rac GTP-Binding Proteins)
EC 3.6.1.- (RAC2 GTP-Binding Protein)
0 (Antioxidants)
0 (Glycosides)
0 (Flavonoids)

Date Created: 20240914 Date Completed: 20240914 Latest Revision: 20241015

20241016

PMC11397025

10.3390/molecules29174182

39275030