Duodenal-Jejunal Bypass Restores Sweet Taste Receptor-Mediated Glucose Sensing and Absorption in Diabetic Rats.

Publisher: Hindawi Limited Country of Publication: England NLM ID: 101605237 Publication Model: eCollection Cited Medium: Internet ISSN: 2314-6753 (Electronic) NLM ISO Abbreviation: J Diabetes Res Subsets: MEDLINE

Publication: <2019>-: London, United Kingdom : Hindawi Limited
Original Publication: Nasr City, Cairo : Hindawi Publishing Corporation, [2013]-

Sodium-Glucose Transporter 1*/metabolism ; Glucose Transporter Type 2*/metabolism ; Jejunum*/surgery ; Jejunum*/metabolism ; Duodenum*/surgery ; Duodenum*/metabolism ; Diabetes Mellitus, Experimental*/surgery ; Diabetes Mellitus, Experimental*/metabolism ; Receptors, G-Protein-Coupled*/metabolism ; Blood Glucose*/metabolism ; Glucagon-Like Peptide 1*/metabolism; Animals ; Male ; Rats ; Intestinal Absorption ; Glucagon-Like Peptide 2/metabolism ; Glucose/metabolism ; Rats, Sprague-Dawley ; Diabetes Mellitus, Type 2/metabolism ; Diabetes Mellitus, Type 2/surgery ; Transducin/metabolism ; Intestinal Mucosa/metabolism ; Intestinal Mucosa/surgery

Aim: The aim of the study is to identify the regulatory role of intestinal sweet taste receptors (STRs) and glucose transporters (SGLT1, GLUT2) and gut peptide secretion in duodenal-jejunal bypass (DJB)-ameliorated glycemic control in Type 2 diabetes. Materials and Methods: DJB and sham surgeries were performed in streptozotocin-induced diabetic male rats. The blood GLP-1 and GLP-2 levels were evaluated under feeding and fasting conditions. The expression of STRs (T1R2, T1R3), sweet taste signaling effector (G α -gustducin), SGLT1, and GLUT2 was detected in the intestinal alimentary limb (A limb), biliopancreatic limb (BP limb), and common limb (C limb). The effects of STR inhibition on glucose control were measured with lactisole. Results: Glucose tolerance was improved in DJB-operated rats compared with the sham group, similar to that of normal control rats, without significant differences in food intake and body weight. The plasma GLP-1 levels of DJB rats were increased under diet-fed condition, and GLP-2 levels were increased after fasting. The villus height and crypt depth were significantly increased in the A limb of DJB-operated rats. In addition, GLP-1 expression was restored in enterocytes. The expression of T1R2, G α -gustducin, and SGLT1 was elevated in the A limb after DJB, while GLUT2 was downregulated in the A, BP, and C limbs. The localization of GLUT2 was normalized in the three intestinal limbs after DJB. However, the beneficial effects of DJB on glucose control were abolished in the presence of lactisole in vivo. Conclusion: DJB ameliorates glycemic control probably by restoring STR-mediated glucose sensing and absorption with the responses of GLP-1 and GLP-2 to carbohydrate.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2024 Sipeng Sun et al.)

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Keywords: DJB; GLP-1; GLUT2; T1R2; lactisole

0 (Sodium-Glucose Transporter 1)
0 (Glucose Transporter Type 2)
0 (Slc5a1 protein, rat)
0 (taste receptors, type 1)
0 (Slc2a2 protein, rat)
0 (Receptors, G-Protein-Coupled)
0 (Blood Glucose)
89750-14-1 (Glucagon-Like Peptide 1)
0 (Glucagon-Like Peptide 2)
IY9XDZ35W2 (Glucose)
147979-21-3 (gustducin)
EC 3.6.5.1 (Transducin)

Date Created: 20240912 Date Completed: 20240912 Latest Revision: 20240914

20240914

PMC11390237

10.1155/2024/5544296

39263491