Cytotoxicity assessment and antimicrobial effects of cell-free supernatants from probiotic lactic acid bacteria and yeast against multi-drug resistant Escherichia coli.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 8510094 Publication Model: Print Cited Medium: Internet ISSN: 1472-765X (Electronic) Linking ISSN: 02668254 NLM ISO Abbreviation: Lett Appl Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2023- : Oxford : Oxford University Press
      Original Publication: Oxford, UK : Published for the Society for Applied Bacteriology by Blackwell Scientific Publications, [c1985-
    • Subject Terms:
      Probiotics*/pharmacology ; Escherichia coli*/drug effects ; Escherichia coli*/genetics ; Saccharomyces cerevisiae*/drug effects ; Saccharomyces cerevisiae*/genetics ; Biofilms*/drug effects ; Drug Resistance, Multiple, Bacterial* ; Microbial Sensitivity Tests* ; Anti-Bacterial Agents*/pharmacology; Humans ; Lactobacillus plantarum/metabolism ; Lactobacillus plantarum/genetics ; HEK293 Cells ; Bifidobacterium bifidum ; Cell Survival/drug effects ; Lactobacillales/metabolism ; Lactobacillales/genetics
    • Abstract:
      The antibacterial, antibiofilm, and cytotoxicity activity of cell-free supernatants (CFSs) from probiotics, including Lactobacillus plantarum, Bifidobacterium bifidum, and Saccharomyces cerevisiae against multi-drug resistant Escherichia coli evaluated in current research. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the CFSs were determined by analyzing inhibition zone formation using agar disk diffusion for antibacterial activity, microtiter plate for biofilm analysis, and auto-aggregation were done. CFSs substances were analyzed by GC-MS. The MTT assay on HEK293 cells investigated CFS's influence on cell viability. CFSs were examined for biofilm-related virulence genes, including aggR and fimH using real-time polymerase chain reaction (real-time PCR). All CFSs had bacteriostatic and bactericidal effects. The B. bifidum exhibited the highest antibiofilm activity compared to the others. Bifidobacterium bifidum, L. plantarum, and S. cerevisiae produce 19, 16, and 11 mm inhibition zones against E. coli, respectively. GC-MS indicated that Hydroxyacetone, 3-Hydroxybutyric acid, and Oxime-methoxy-phenyl-dominated CFSs from L. plantarum, B. bifidum, and S. cerevisiae CFSs, respectively. The MTT test demonstrated a cell viability rate of over 90%. Statistically, adding all CFSs lowered the relative expression of both aggR and fimH virulence genes.
      (© The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.)
    • Grant Information:
      70277 Tabriz University of Medical Sciences
    • Contributed Indexing:
      Keywords: antibacterial activity; antibiofilm activity; cell-free supernatants (CFSs); drug-resistant; lactic acid bacteria; probiotics
    • Accession Number:
      0 (Anti-Bacterial Agents)
    • Publication Date:
      Date Created: 20240905 Date Completed: 20240914 Latest Revision: 20240914
    • Publication Date:
      20240914
    • Accession Number:
      10.1093/lambio/ovae084
    • Accession Number:
      39237462