Investigate the in vitro biocompatibility, biodegradation, cytotoxicity, and differentiation potential of 3-D gelatin-nanocellulose composite scaffolds loaded with nanohydroxyapatite and simvastatin.

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  • Additional Information
    • Source:
      Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 0214745 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-3072 (Electronic) Linking ISSN: 00408166 NLM ISO Abbreviation: Tissue Cell Subsets: MEDLINE
    • Publication Information:
      Publication: Edinburgh : Churchill Livingstone
      Original Publication: Edinburgh, Oliver & Boyd.
    • Subject Terms:
      Simvastatin*/pharmacology ; Simvastatin*/chemistry ; Gelatin*/chemistry ; Gelatin*/pharmacology ; Tissue Scaffolds*/chemistry ; Durapatite*/chemistry ; Durapatite*/pharmacology ; Cellulose*/chemistry ; Cellulose*/pharmacology ; Cell Differentiation*/drug effects ; Mesenchymal Stem Cells*/drug effects ; Mesenchymal Stem Cells*/metabolism ; Mesenchymal Stem Cells*/cytology; Humans ; Tissue Engineering ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Nanocomposites/chemistry ; Materials Testing ; Osteogenesis/drug effects
    • Abstract:
      Bone tissue engineering has been proposed as a promising solution for healing of bone fractures. An important aspect of bone tissue engineering is the implantable scaffolds that participate in the regeneration and repair of bone tissue. In this study, the composite scaffolds of gelatin- nanocellulose loaded with nanohydroxyapatite and simvastatin (as the osteoinductive component) were fabricated using freeze- drying method. Scaffolds were characterized in terms of morphology, mechanical, biodegradability, water absorption capacity, and simvastatin release characteristics. Also, the biocompatibility and differentiation potential of the scaffolds were evaluated on human bone marrow-derived mesenchymal stem cells using the MTT assay and alizarin red staining, respectively. The simvastatin loaded scaffolds showed a sustained release profile in vitro up to 216 h. The results of BMSCs differentiation by alizarin red staining showed significant differences between the simvastatin loaded group and other groups. Moreover, the results of MTT assay verified cytocompatibility and non-toxicity of the scaffolds. Therefore, the gelatin-nano cellulose composite scaffolds loaded with hydroxyapatite and simvastatin may be considered promising for use in bone tissue engineering.
      Competing Interests: Declaration of Competing Interest The authors declare that there are no conflicts of interest.
      (Copyright © 2024 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: Bone fractures; Bone marrow-derived mesenchymal stem cells; Bone tissue engineering; Gelatin; Nanocellulose; Nanohydroxyapatite
    • Accession Number:
      AGG2FN16EV (Simvastatin)
      9000-70-8 (Gelatin)
      91D9GV0Z28 (Durapatite)
      9004-34-6 (Cellulose)
      0 (Biocompatible Materials)
    • Publication Date:
      Date Created: 20240905 Date Completed: 20241208 Latest Revision: 20241208
    • Publication Date:
      20241210
    • Accession Number:
      10.1016/j.tice.2024.102536
    • Accession Number:
      39236521