Flow Cytometry and Automated Microscopy Integration for Phagocytic Analysis of Hypervirulent Klebsiella pneumoniae in Dictyostelium discoideum.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Totowa, NJ : Humana Press
      Original Publication: Clifton, N.J. : Humana Press,
    • Subject Terms:
      Dictyostelium*/microbiology ; Flow Cytometry*/methods ; Klebsiella pneumoniae*/pathogenicity; Phagocytosis ; Virulence ; Host-Pathogen Interactions ; Microscopy/methods
    • Abstract:
      This chapter introduces protocols for culturing and maintaining Dictyostelium discoideum and methods for conducting virulence assays in this organism to study bacterial pathogenicity. It outlines advanced techniques, such as automated microscopy and flow cytometry, for detailed cellular analysis and traditional microbiological approaches. These comprehensive protocols will enable researchers to probe the virulence factors of pathogens like Klebsiella pneumoniae and to elucidate the details of host-pathogen interactions within a cost-effective and adaptable laboratory framework.
      (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
    • References:
      Xu X, Pan M, Jin T (2021) How phagocytes acquired the capability of hunting and removing pathogens from a human body: lessons learned from chemotaxis and phagocytosis of Dictyostelium discoideum (review). Front Cell Dev Biol 9:724940. (PMID: 10.3389/fcell.2021.724940344902718417749)
      Froquet R, Lelong E, Marchetti A, Cosson P (2009) Dictyostelium discoideum: a model host to measure bacterial virulence. Nat Protoc 4:25–30. https://doi.org/10.1038/nprot.2008.212. (PMID: 10.1038/nprot.2008.21219131953)
      Bravo-Toncio C, Álvarez JA, Campos F, Ortíz-Severín J, Varas M, Cabrera R, Lagos CF, Chávez FP (2016) Dictyostelium discoideum as a surrogate host–microbe model for antivirulence screening in Pseudomonas aeruginosa PAO1. Int J Antimicrob Agents:1–7. https://doi.org/10.1016/j.ijantimicag.2016.02.005.
      Marcoleta AE, Varas MA, Ortiz-Severín J, Vásquez L, Berríos-Pastén C, Sabag AV, Chávez FP, Allende ML, Santiviago CA, Monasterio O et al (2018) Evaluating different virulence traits of Klebsiella pneumoniae using Dictyostelium discoideum and zebrafish larvae as host models. Front Cell Infect Microbiol 8. https://doi.org/10.3389/fcimb.2018.00030.
    • Contributed Indexing:
      Keywords: Dictyostelium discoideum; Host-pathogen interaction; Hypervirulent Klebsiella pneumoniae; Protocol; Virulence
    • Publication Date:
      Date Created: 20240905 Date Completed: 20240905 Latest Revision: 20240905
    • Publication Date:
      20240905
    • Accession Number:
      10.1007/978-1-0716-4100-2_13
    • Accession Number:
      39235745