Scaffold loaded LPS-hUCMSC-sEVs promote Osteo/odontogenic differentiation and angiogenic potential of hDPSCs.

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  • Additional Information
    • Source:
      Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 0214745 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-3072 (Electronic) Linking ISSN: 00408166 NLM ISO Abbreviation: Tissue Cell Subsets: MEDLINE
    • Publication Information:
      Publication: Edinburgh : Churchill Livingstone
      Original Publication: Edinburgh, Oliver & Boyd.
    • Subject Terms:
      Dental Pulp*/cytology ; Dental Pulp*/metabolism ; Cell Differentiation*/drug effects ; Lipopolysaccharides*/pharmacology ; Tissue Scaffolds*/chemistry ; Mesenchymal Stem Cells*/metabolism ; Mesenchymal Stem Cells*/cytology ; Mesenchymal Stem Cells*/drug effects ; Osteogenesis*/drug effects ; Neovascularization, Physiologic*/drug effects ; Extracellular Vesicles*/metabolism ; Odontogenesis*/drug effects; Humans ; Nanofibers/chemistry
    • Abstract:
      Objective: The formation of dentin-pulp complex determines the success of vital pulp therapy. Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles (hUCMSC-sEVs) appeared to have stronger effect in anti-inflammatory and promoting the proliferation and migration of human dental pulp stem cells (hDPSCs). Moreover, Lipopolysaccharides (LPS) pretreatment can enhance the rapeutic potency of extracellular vesicles. LPS pretreatment hUCMSC-sEVs have the potential to regenerate the dentin-pulp complex by recruiting hDPSCs. This paper aims to develop collagen sponge/self-assembling peptide nanofiber scaffold (CS/SAPNS) composite scaffold loaded with LPS pretreatment hUCMSC-sEVs (CS/SAPNS-sEVs), and assess the release characteristics of hUCMSC-sEVs and the effect of this composite scaffold on osteo/odontogenic differentiation and angiogenic potential in hDPSCs.
      Methods: LPS pretreatment hUCMSC-sEVs (LPS-hUCMSC-sEVs) were mixed with self-assembling peptide hydrogel and loaded onto collagen sponge to obtain the CS/SAPNS-sEVs. BCA assay, nanoparticle analysis, transmission electron microscopy and laser confocal microscopy were used to investigate the characteristics of LPS-hUCMSC-sEVs loaded on CS/SAPNS. Osteo/odontogenic differentiation ability of hDPSCs were analyzed by ALP stainning, alizarin red staining. RT-PCR and Western blot analysis were performed to confirm the levels of osteo/odontogenic factors and angiogenic factors, and the involvement of NF-κB pathway was verified by immunocytochemical staining and Western blot analysis.
      Results: CS/SAPNS could control LPS-hUCMSC-sEVs release for 7 days and keep their structural integrity. CS/SAPNS-sEVs promoted deposition of calcified nodules and expression of osteogenic/odontogenic and angiogenic factors in hDPSCs. On the contrary, inhibition of the NF-κB pathway down-regulated the expression of CS/SAPNS-sEVs-regulated osteo/odontogenic and angiogenic factors.
      Conclusion: CS/SNAPS could be used as scaffold for LPS-hUCMSC-sEVs, and CS/SAPNS-sEVs may promote osteo/odontogenic differentiation and enhance the angiogenic potential of hDPSCs through activating the NF-κB pathway.
      Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
      (Copyright © 2024 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: Dental pulp stem cells; NF-κB pathway; Osteo/odontogenic differentiation; Scaffold; Small extracellular vesicles
    • Accession Number:
      0 (Lipopolysaccharides)
    • Publication Date:
      Date Created: 20240903 Date Completed: 20241208 Latest Revision: 20241208
    • Publication Date:
      20241210
    • Accession Number:
      10.1016/j.tice.2024.102549
    • Accession Number:
      39226663