RNA-seq reveals the important role of transcriptional regulator DeoR1 in regulating Brucella abortus various pathways.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101475056 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7737 (Electronic) Linking ISSN: 18743919 NLM ISO Abbreviation: J Proteomics Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam : Elsevier
    • Subject Terms:
      Brucella abortus*/genetics ; Brucella abortus*/metabolism ; Brucella abortus*/pathogenicity ; Gene Expression Regulation, Bacterial* ; RNA-Seq* ; Bacterial Proteins*/genetics ; Bacterial Proteins*/metabolism; Animals ; Mice ; RAW 264.7 Cells ; Virulence/genetics ; Brucellosis/microbiology ; Brucellosis/genetics ; Brucellosis/metabolism ; Transcription Factors/metabolism ; Transcription Factors/genetics ; Macrophages/microbiology ; Macrophages/metabolism
    • Abstract:
      Brucella spp. is an intracellular bacterium that uses its transcriptional regulator DeoR1 to promote intracellular transport and survival, but the molecular mechanism remains unknown. To analyze the role of DeoR1 in the virulence of B. abortus and the genes regulated by DeoR1, we created a A19ΔdeoR1 mutant of B. abortus A19 (A19). Virulence assay was performed using a murine macrophage cell line (RAW264.7) and mice. We observed that A19ΔdeoR1 mutant is attenuated in RAW264.7 cells and mice. We performed RNA-seq whole transcriptome analysis of A19ΔdeoR1 and A19 from infected RAW264.7 cells. A total of 135 differentially expressed genes were identified, including 100 up-regulated and 35 down-regulated genes. These differentially expressed genes were involved in amino acid synthesis and metabolism, energy production and conversion, stress proteins, chaperonin, hypothetical proteins and protein of unknown function, cell wall/membrane/envelope, intracellular transporting and secretion, and transcriptional regulator. Interestingly, genes involved in the intracellular trafficking and secretion were significantly down-regulated in A19ΔdeoR1. Furthermore, selected RNA-seq results were experimentally confirmed by qRT-PCR. Overall, these results deciphered differential phenomena associated with virulence in A19ΔdeoR1 and A19 from infected RAW264.7 cells, which provided important information for understanding the detailed role of DeoR1 in Brucella pathogenesis. SIGNIFICANCE: Transcriptional regulators are predominant bacterial signal transduction factors. The pathogenicity of Brucella is due to its ability to regulate the expression of virulence related genes. Transcriptional regulators are designed to regulate gene expression and enact an appropriate adaptive physiological response. Here, a total of 135 differentially expressed genes were identified in transcriptional regulator deoR1 mutant.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024. Published by Elsevier B.V.)
    • Contributed Indexing:
      Keywords: Brucella; DeoR1; RNA-seq
    • Accession Number:
      0 (Bacterial Proteins)
      0 (Transcription Factors)
    • Publication Date:
      Date Created: 20240830 Date Completed: 20241027 Latest Revision: 20241104
    • Publication Date:
      20241104
    • Accession Number:
      10.1016/j.jprot.2024.105297
    • Accession Number:
      39214508