Integrin-β 1 aggravates paraquat-induced pulmonary fibrosis by activation of FAK/ ERK1/2 pathway depending on fibrotic ECM.

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  • Additional Information
    • Source:
      Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 100965259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1705 (Electronic) Linking ISSN: 15675769 NLM ISO Abbreviation: Int Immunopharmacol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam ; New York : Elsevier Science, c2001-
    • Subject Terms:
      Paraquat*/toxicity ; Pulmonary Fibrosis*/chemically induced ; Pulmonary Fibrosis*/pathology ; Pulmonary Fibrosis*/metabolism ; Extracellular Matrix*/metabolism ; Fibroblasts*/drug effects ; Fibroblasts*/pathology ; Fibroblasts*/metabolism ; Integrin beta1*/metabolism ; MAP Kinase Signaling System*/drug effects ; Rats, Sprague-Dawley*; Animals ; Male ; Rats ; Focal Adhesion Kinase 1/metabolism ; Lung/pathology ; Lung/drug effects ; Cell Differentiation/drug effects ; Myofibroblasts/metabolism ; Myofibroblasts/pathology ; Myofibroblasts/drug effects ; Cells, Cultured ; Disease Models, Animal
    • Abstract:
      Background: Irreversible pulmonary fibrosis induced by paraquat is the most prevalent cause of death in patients with paraquat poisoning. Pulmonary fibrosis is characterized by abnormal deposition of extracellular matrix (ECM). Currently, the role of fibrotic ECM microenvironment in paraquat-induced pulmonary fibrosis has not been established.
      Methods: Rat pulmonary fibrosis model was induced by paraquat, ATN-161 (an integrin-β 1 antagonist) was given to investigate their effect on Rat survival and pulmonary fibrosis. Lungs were decellularized to generate normal and fibrotic acellular ECM scaffolds using Triton and SDS. Fibroblasts were cocultured with ECM scaffolds to established 3D culture systems to investigate the relationship between fibrotic ECM and the differentiation of fibroblasts. Then we explored the effect of fibrotic ECM microenvironment systematically promoting on integrin-β1/FAK/ERK1/2 pathway and established 3D culture systems to investigate the relationship between fibrotic ECM and the differentiation of fibroblasts.
      Results: Antagonism of integrin-β 1 could alleviate paraquat-induced pulmonary fibrosis and ameliorate survival status of rats. Compared to normal ECM, fibrotic extracellular microenvironment promoted the differentiation of fibroblasts to myofibroblasts. Antagonism of integrin-β 1 could also ameliorate the promotion of fibrotic extracellular microenvironment on differentiation of fibroblasts to myofibroblasts. Fibrotic ECM microenvironment promotes fibroblasts transforming into myofibroblasts through integrin-β 1 /FAK/ERK1/2 signaling pathway. Moreover, this phenomenon holds independent on exogenous integrin-β 1 .
      Conclusions: Activation of integrin-β 1 /FAK/ERK1/2 pathway aggravates paraquat-induced pulmonary fibrosis depend on fibrotic ECM and integrin-β 1 may be a prospective therapeutic target for paraquat-induced pulmonary fibrosis in the future.
      Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024. Published by Elsevier B.V.)
    • Contributed Indexing:
      Keywords: Extracellular Matrix; Integrin-β(1); Myofibroblast; Paraquat; Pulmonary Fibrosis
    • Accession Number:
      PLG39H7695 (Paraquat)
      0 (Integrin beta1)
      EC 2.7.10.2 (Focal Adhesion Kinase 1)
      EC 2.7.10.2 (Ptk2 protein, rat)
    • Publication Date:
      Date Created: 20240830 Date Completed: 20240917 Latest Revision: 20241112
    • Publication Date:
      20241112
    • Accession Number:
      10.1016/j.intimp.2024.112947
    • Accession Number:
      39213871