Genomic analysis of two penicillin- and rifampin-resistant Corynebacterium rouxii strains isolated from cutaneous infections in dogs.

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  • Additional Information
    • Source:
      Publisher: British Veterinary Association Country of Publication: England NLM ID: 0401300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2661 (Electronic) Linking ISSN: 00345288 NLM ISO Abbreviation: Res Vet Sci Subsets: MEDLINE
    • Publication Information:
      Publication: London : British Veterinary Association
      Original Publication: Oxford.
    • Subject Terms:
      Corynebacterium*/genetics ; Corynebacterium*/drug effects ; Dog Diseases*/microbiology ; Rifampin*/pharmacology ; Corynebacterium Infections*/veterinary ; Corynebacterium Infections*/microbiology ; Phylogeny* ; Anti-Bacterial Agents*/pharmacology; Animals ; Dogs ; Genome, Bacterial ; Drug Resistance, Bacterial/genetics ; Penicillins/pharmacology
    • Abstract:
      Although diphtheria is a vaccine-preventable disease, numerous cases are still reported around the world, as well as outbreaks in countries, including European ones. Species of the Corynebacterium diphtheriae complex are potentially toxigenic and, therefore, must be considered given the possible consequences, such as the circulation of clones and transmission of antimicrobial resistance and virulence genes. Recently, Corynebacterium rouxii was characterized and included among the valid species of the complex. Therefore, two cases of C. rouxii infection arising from infections in domestic animals are presented here. We provide molecular characterization, phylogenetic analyses, genome sequencing, and CRISPR-Cas analyses to contribute to a better understanding of the molecular bases, pathogenesis, and epidemiological monitoring of this species, which is still little studied. We confirmed its taxonomic position with genome sequencing and in silico analysis and identified the ST-918 for both strains. The clinical isolates were sensitive resistance to benzylpenicillin and rifampin. Antimicrobial resistance genes, including tetB, rpoB2, and rbpA genes, were predicted. The bla and ampC genes were not found. Several virulence factors were also detected, including adhesion, iron uptake systems, gene regulation (dtxR), and post-translational modification (MdbA). Finally, one prophage and the Type I-E CRISPR-Cas system were identified.
      Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.
      (Copyright © 2024 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: CRISPR-Cas system; Corynebacterium diphtheriae complex; Corynebacterium rouxii; Non-toxigenic; Resistance genes; Virulence factors
    • Accession Number:
      VJT6J7R4TR (Rifampin)
      0 (Anti-Bacterial Agents)
      0 (Penicillins)
    • Publication Date:
      Date Created: 20240830 Date Completed: 20240910 Latest Revision: 20240910
    • Publication Date:
      20240911
    • Accession Number:
      10.1016/j.rvsc.2024.105396
    • Accession Number:
      39213744