Atriplex hortensis var. 'rubra' extracts and purified amaranthin-type pigments reduce oxidative stress and inflammatory response in LPS-stimulated RAW264.7 cells.

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  • Additional Information
    • Source:
      Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE
    • Publication Information:
      Publication: Barking : Elsevier Applied Science Publishers
      Original Publication: Barking, Eng., Applied Science Publishers.
    • Subject Terms:
      Plant Extracts*/pharmacology ; Plant Extracts*/chemistry ; Plant Extracts*/isolation & purification ; Oxidative Stress*/drug effects ; Macrophages*/drug effects ; Macrophages*/immunology ; Lipopolysaccharides*/pharmacology; Mice ; Animals ; RAW 264.7 Cells ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/chemistry ; Anti-Inflammatory Agents/isolation & purification ; Cyclooxygenase 2/genetics ; Cyclooxygenase 2/immunology ; Cyclooxygenase 2/metabolism ; Inflammation/drug therapy ; Inflammation/metabolism ; Tandem Mass Spectrometry
    • Abstract:
      The use of direct injection ion mobility mass spectrometry (DI-IM-MS) to detect and identify betacyanin pigments in A. hortensis 'rubra' extracts was explored for the first time, with results compared to conventional LC-MS/MS analysis. The anti-inflammatory activities of leaf and seed extracts, alongside purified amaranthin and celosianin pigments, were investigated using a model of lipopolysaccharide (LPS)-activated murine macrophages. Extracts and purified pigments significantly inhibited the production of prostaglandin E 2 and NO by up to 90% and 70%, respectively, and reduced the expression of Il6, Il1b, Nos2, and Cox2. Leaf and seed extracts also decreased secretion of Il6 and Il1b cytokines and reduced protein levels of Nos2 and Cox2. Furthermore, extracts and purified pigments demonstrated potent dose-dependent radical scavenging activity in a cellular antioxidant activity assay (CAA) without any cytotoxic effects. Our research highlights the promising biological potential of edible, climate-resilient A. hortensis 'rubra' as a valuable source of bioactive compounds.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
    • Contributed Indexing:
      Keywords: Anti-inflammatory activity; Antioxidant activity; Atriplex hortensis ‘rubra’; Betalains; Ion mobility-mass spectrometry
    • Accession Number:
      0 (Plant Extracts)
      0 (Lipopolysaccharides)
      0 (Anti-Inflammatory Agents)
      EC 1.14.99.1 (Cyclooxygenase 2)
    • Publication Date:
      Date Created: 20240829 Date Completed: 20240912 Latest Revision: 20240912
    • Publication Date:
      20240913
    • Accession Number:
      10.1016/j.foodchem.2024.140920
    • Accession Number:
      39208732