Human Skeletal Muscle Myoblast Culture in Aligned Bacterial Nanocellulose and Commercial Matrices.

Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE

Original Publication: Washington, D.C. : American Chemical Society

Cellulose*/chemistry ; Tissue Scaffolds*/chemistry ; Tissue Engineering*; Humans ; Myoblasts, Skeletal/metabolism ; Myoblasts, Skeletal/cytology ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Muscle, Skeletal/cytology ; Muscle, Skeletal/chemistry ; Cells, Cultured ; Myoblasts/cytology ; Nanostructures/chemistry ; Acetobacteraceae/chemistry ; Acetobacteraceae/metabolism ; Hydrogels/chemistry

Bacterial nanocellulose (BNC) is a durable, flexible, and dynamic biomaterial capable of serving a wide variety of fields, sectors, and applications within biotechnology, healthcare, electronics, agriculture, fashion, and others. BNC is produced spontaneously in carbohydrate-rich bacterial culture media, forming a cellulosic pellicle via a nanonetwork of fibrils extruded from certain genera. Herein, we demonstrate engineering BNC-based scaffolds with tunable physical and mechanical properties through postprocessing. Human skeletal muscle myoblasts (HSMMs) were cultured on these scaffolds, and in vitro electrical stimulation was applied to promote cellular function for tissue engineering applications. We compared physiologic maturation markers of human skeletal muscle myoblast development using a 2.5-dimensional culture paradigm in fabricated BNC scaffolds, compared to two-dimensional (2D) controls. We demonstrate that the culture of human skeletal muscle myoblasts on BNC scaffolds developed under electrical stimulation produced highly aligned, physiologic morphology of human skeletal muscle myofibers compared to unstimulated BNC and standard 2D culture. Furthermore, we compared an array of metrics to assess the BNC scaffold in a rigorous head-to-head study with commercially available, clinically approved matrices, Kerecis Omega3 Wound Matrix (Marigen) and Phoenix as well as a gelatin methacryloyl (GelMA) hydrogel. The BNC scaffold outcompeted industry standard matrices as well as a 20% GelMA hydrogel in durability and sustained the support of human skeletal muscle myoblasts in vitro. This work offers a robust demonstration of BNC scaffold cytocompatibility with human skeletal muscle cells and sets the basis for future work in healthcare, bioengineering, and medical implant technological development.

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T32 AR065972 United States AR NIAMS NIH HHS

Keywords: aligned; bacterial nanocellulose; bioreactor; electrically stimulated; epitaxial; human skeletal muscle myoblasts; hydrogel; mesh; soft-tissue reconstruction

9004-34-6 (Cellulose)
0 (Biocompatible Materials)
0 (Hydrogels)

Date Created: 20240829 Date Completed: 20240912 Latest Revision: 20240918

20240918

PMC11403597

10.1021/acsami.4c07612

39206938