Ciliary Motility Decreased by a CO 2 /HCO 3 - -Free Solution in Ciliated Human Nasal Epithelial Cells Having a pH Elevated by Carbonic Anhydrase IV.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • Subject Terms:
      Carbon Dioxide*/metabolism ; Cilia*/metabolism ; Bicarbonates*/metabolism ; Epithelial Cells*/metabolism ; Nasal Mucosa*/metabolism ; Nasal Mucosa*/cytology ; Carbonic Anhydrase IV*/metabolism ; Carbonic Anhydrase IV*/genetics; Humans ; Hydrogen-Ion Concentration ; Cells, Cultured ; Sodium-Bicarbonate Symporters/metabolism ; Sodium-Bicarbonate Symporters/genetics
    • Abstract:
      An application of CO 2 /HCO 3 - -free solution (Zero-CO 2 ) did not increase intracellular pH (pH i ) in ciliated human nasal epithelial cells (c-hNECs), leading to no increase in frequency (CBF) or amplitude (CBA) of the ciliary beating. This study demonstrated that the pH i of c-hNECs expressing carbonic anhydrase IV (CAIV) is high (7.64), while the pH i of ciliated human bronchial epithelial cells (c-hBECs) expressing no CAIV is low (7.10). An extremely high pH i of c-hNECs caused pH i , CBF and CBA to decrease upon Zero-CO 2 application, while a low pH i of c-hBECs caused them to increase. An extremely high pH i was generated by a high rate of HCO 3 - influx via interactions between CAIV and Na + /HCO 3 - cotransport (NBC) in c-hNECs. An NBC inhibitor (S0859) decreased pH i , CBF and CBA and increased CBF and CBA in c-hNECs upon Zero-CO 2 application. In conclusion, the interactions of CAIV and NBC maximize HCO 3 - influx to increase pH i in c-hNECs. This novel mechanism causes pH i to decrease, leading to no increase in CBF and CBA in c-hNECs upon Zero-CO 2 application, and appears to play a crucial role in maintaining pH i , CBF and CBA in c-hNECs periodically exposed to air (0.04% CO 2 ) with respiration.
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    • Grant Information:
      JP18H03182 Japan Society for the Promotion of Science
    • Contributed Indexing:
      Keywords: ALI; CBF; NBC; NH4+ pulse; airway; bicarbonate transport metabolon; ciliary motility; ciliary waveform; intracellular pH; nasal cilia
    • Accession Number:
      142M471B3J (Carbon Dioxide)
      0 (Bicarbonates)
      EC 4.2.1.- (Carbonic Anhydrase IV)
      0 (Sodium-Bicarbonate Symporters)
    • Publication Date:
      Date Created: 20240829 Date Completed: 20240829 Latest Revision: 20240903
    • Publication Date:
      20240903
    • Accession Number:
      PMC11354224
    • Accession Number:
      10.3390/ijms25169069
    • Accession Number:
      39201753