Functional Characterization of an Odorant Receptor Expressed in Newly Hatched Larvae of Fall Armyworm Spodoptera frugiperda .

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  • Additional Information
    • Source:
      Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101574235 Publication Model: Electronic Cited Medium: Print ISSN: 2075-4450 (Print) Linking ISSN: 20754450 NLM ISO Abbreviation: Insects Subsets: PubMed not MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI AG, [2010]-
    • Abstract:
      In the past decade, Spodoptera frugiperda has emerged as a significant invasive pest globally, posing a serious threat to agriculture due to its broad diet, migratory behavior, and ability to cause extensive plant damage. While extensive research has focused on the olfactory capabilities of adult S. frugiperda , understanding of the olfactory process in larvae remains limited, despite larvae playing a crucial role in crop damage. To address this gap, we identified an odorant receptor (OR), SfruOR40, expressed in the first-instar larvae through phylogenetic analysis. Using quantitative real-time PCR, we compared SfruOR40 expression levels in larvae and adults. We then characterized the function of SfruOR40 against 67 compounds using the Xenopus oocyte expression system and found that SfruOR40 responded to three plant volatiles. Further, behavioral experiments revealed a larval attraction to (-)- trans -Caryophyllene oxide. This study elucidates SfruOR40's role in the olfactory recognition of newly hatched S. frugiperda larvae, expanding our knowledge of such mechanisms in Noctuid moths. Furthermore, it highlights the potential of plant-derived natural products for biological pest control from a behavioral ecology perspective.
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    • Grant Information:
      2023YFE0104800 National Key R&D Program of China; 32272540, 32072509 National Natural Science Foundation of China; CAAS-CSCB-202302 Innovation Program of Chinese Academy of Agricultural Sciences
    • Contributed Indexing:
      Keywords: Spodoptera frugiperda; behavior; newly hatched larvae; odorant receptor; plant volatiles
    • Publication Date:
      Date Created: 20240828 Latest Revision: 20240830
    • Publication Date:
      20250114
    • Accession Number:
      PMC11354698
    • Accession Number:
      10.3390/insects15080564
    • Accession Number:
      39194769