Identifying an Abnormal Phosphorylated Adaptor by Viral Kinase Using Mass Spectrometry.

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  • Author(s): Su C;Su C; Su C; Su C; Zheng C; Zheng C
  • Source:
    Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2854, pp. 29-34.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Totowa, NJ : Humana Press
      Original Publication: Clifton, N.J. : Humana Press,
    • Subject Terms:
      Tandem Mass Spectrometry*/methods ; Immunoprecipitation*/methods ; Phosphoproteins*/metabolism ; Phosphoproteins*/analysis; Phosphorylation ; Chromatography, Liquid/methods ; Humans ; Mass Spectrometry/methods
    • Abstract:
      Mass spectrometers are widely used to identify protein phosphorylation sites. The process usually involves selective isolation of phosphoproteins and subsequent fragmentation to identify both the peptide sequence and phosphorylation site. Immunoprecipitation could capture and purify the protein of interest, greatly reducing sample complexity before submitting it for mass spectrometry analysis. This chapter describes a method to identify an abnormal phosphorylated site of the adaptor protein by a viral kinase through immunoprecipitation followed by LC-MS/MS.
      (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
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    • Contributed Indexing:
      Keywords: A signaling adaptor; Immunoprecipitation; LC-MS/MS; Phosphorylation; Viral kinase
    • Accession Number:
      0 (Phosphoproteins)
    • Publication Date:
      Date Created: 20240827 Date Completed: 20240827 Latest Revision: 20240827
    • Publication Date:
      20240828
    • Accession Number:
      10.1007/978-1-0716-4108-8_4
    • Accession Number:
      39192115