Corpus luteum proximity alters molecular signature of the small extracellular vesicles and cumulus cells in the bovine ovarian follicle environment.

Publisher: North Holland Publishing Country of Publication: Ireland NLM ID: 7500844 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8057 (Electronic) Linking ISSN: 03037207 NLM ISO Abbreviation: Mol Cell Endocrinol Subsets: MEDLINE

Publication: Limerick : North Holland Publishing
Original Publication: Amsterdam, North-Holland.

Cumulus Cells*/metabolism ; Cumulus Cells*/cytology ; Extracellular Vesicles*/metabolism ; Extracellular Vesicles*/genetics ; Corpus Luteum*/metabolism ; Corpus Luteum*/cytology ; Progesterone*/metabolism ; Ovarian Follicle*/metabolism ; Ovarian Follicle*/cytology; Animals ; Female ; Cattle ; Oocytes/metabolism ; Cell Communication

Progesterone (P4) is predicted to act as a negative regulatory hormone for oocyte maturation events; however, its local effects during follicular development remain poorly understood in bovine. The complex process of oocyte meiosis progression is dependent on cellular communication among follicular cells. Besides, the breakdown of this communication, mainly between cumulus cells (CC) and oocyte, through the retraction of cumulus projections connecting these cells can impact oocyte maturation. In our study, we observed that follicles from the ovary ipsilateral to the corpus luteum (CL) containing high intrafollicular P4 concentrations enhance the abundance of proteins detected in follicular-derived small extracellular vesicles (sEVs) predicted to be involved in the retraction of membrane projections based on actin filaments, such as transzonal projections (TZPs). Conversely, we found that follicles from the ovary contralateral to the CL, which contained low intrafollicular P4 concentrations, had a high detection of proteins predicted to regulate the maintenance of TZPs. We also performed RNAseq analysis which demonstrated that 177 genes were differentially expressed in CC under the different P4 environments. Bioinformatic analysis points to changes associated to cell metabolism in cells from follicles ipsilateral to the CL in comparison to genes involved in cell communication in CC from follicles contralateral to the CL. Our functional analysis experiment confirmed that supplementation of cumulus-oocyte complexes during in vitro maturation with P4 at concentration similar to ipsilateral follicles reduces the number of TZPs. In summary, our study underscores a direct association between P4 concentration and cumulus-oocyte interaction, with potential consequences for the acquisition of oocyte competence.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Juliano Coelho da Silveira reports financial support was provided by São Paulo Research Foundation - FAPESP. Juliano Coelho da Silveira reports financial support was provided by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES). Juliano Coelho da Silveira reports financial support was provided by National Council for Scientific and Technological Development. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)

Keywords: Follicular development; Oocyte maturation; Small extracellular vesicles; Transzonal projections

4G7DS2Q64Y (Progesterone)

Date Created: 20240824 Date Completed: 20240907 Latest Revision: 20240907

20240908

10.1016/j.mce.2024.112347

39181310