Comparative Evaluation of Micellization and Cellular Uptake of β -Carotene Affected by Flavonoids.

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  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, American Chemical Society.
    • Subject Terms:
      beta Carotene*/metabolism ; beta Carotene*/chemistry ; Flavonoids*/metabolism ; Flavonoids*/chemistry ; Micelles* ; Citrus*/chemistry ; Citrus*/metabolism; Humans ; Caco-2 Cells ; Biological Transport ; Digestion ; Flavanones/metabolism ; Flavanones/chemistry ; Rutin/metabolism ; Rutin/chemistry ; Plant Extracts/metabolism ; Plant Extracts/chemistry ; Membrane Transport Proteins
    • Abstract:
      Based on in vitro digestion, micellar synthesis, and Caco-2 cell model, this study investigated the effects of typical flavonoids in citrus (naringenin, naringin, hesperetin, hesperidin, quercetin, and rutin) at different doses on the micellization and cellular uptake of β -carotene. In in vitro digestion, low-dose flavonoids enhanced β -carotene bioaccesssibility by regulating the stability and dispersibility of the intestinal medium, particularly quercetin, which significantly increased the bioaccessibility by 44.6% ( p < 0.05). Furthermore, naringenin, hesperetin, hesperidin, and quercetin enhanced the micellar incorporation rate of β -carotene; however, naringin and rutin exhibited an opposite effect, particularly naringin, which significantly reduced it by 71.3% ( p < 0.05). This phenomenon could be attributed to the high solubility of naringin and rutin in micelles, resulting in a competitive inhibitory effect on β -carotene. Besides, all treatments significantly enhanced β -carotene cellular uptake ( p < 0.05) by promoting the expression of scavenger receptor class B type I and Niemann-Pick C1-Like 1.
    • Contributed Indexing:
      Keywords: Caco-2 cell; bioaccessibility; flavonoids; micellar incorporation rate; β-carotene
    • Accession Number:
      01YAE03M7J (beta Carotene)
      0 (Flavonoids)
      0 (Micelles)
      0 (Flavanones)
      0 (NPC1L1 protein, human)
      N7TD9J649B (naringin)
      5G06TVY3R7 (Rutin)
      HN5425SBF2 (naringenin)
      0 (Plant Extracts)
      0 (Membrane Transport Proteins)
    • Publication Date:
      Date Created: 20240822 Date Completed: 20240904 Latest Revision: 20240904
    • Publication Date:
      20240904
    • Accession Number:
      10.1021/acs.jafc.4c03554
    • Accession Number:
      39174497