Influence of inhibiting methemoglobin formation on erythrocyte antioxidant defense.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0372430 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0384 (Electronic) Linking ISSN: 00039861 NLM ISO Abbreviation: Arch Biochem Biophys Subsets: MEDLINE
    • Publication Information:
      Publication: <2000- > : San Diego, CA : Elsevier
      Original Publication: New York, NY : Academic Press
    • Subject Terms:
      Methemoglobin*/metabolism ; Erythrocytes*/metabolism ; Peroxiredoxins*/metabolism ; Antioxidants*/metabolism ; Glutathione Peroxidase*/metabolism ; Catalase*/metabolism ; Oxidative Stress*; Humans ; Hydrogen Peroxide/metabolism ; Oxidation-Reduction ; Cytosol/metabolism ; Male ; Adult
    • Abstract:
      We aimed to study the influence of preventing methemoglobin (metHb) formation, in the roles of peroxiredoxin 2 (Prx2), glutathione peroxidase (GPx) and catalase (CAT) on the erythrocyte antioxidant defense system. We performed in vitro assays using healthy erythrocytes, with and without inhibition of autoxidation of Hb (saturation with carbon monoxide), followed by H 2 O 2 -induced oxidative stress. We assessed the enzyme activities and amounts of CAT, GPx and Prx2 in the red blood cell (RBC) cytosol and membrane and several biomarkers of oxidative stress, such as the reduced and oxidized glutathione levels, thiobarbituric acid reactive substances (TBARS) levels, membrane bound hemoglobin and total antioxidant status. When autoxidation of Hb was inhibited, no significant changes were found for GPx and CAT; Prx2 was observed only in the monomeric form in the cytosol and none bound to the membrane. Blocking the function of Hb as a pseudo-peroxidase does not seem to have an impact on the function of the RBC peroxidases.
      (Copyright © 2024 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Hemoglobin; Oxidative stress; Peroxiredoxin 2; Red blood cell
    • Accession Number:
      9008-37-1 (Methemoglobin)
      EC 1.11.1.15 (Peroxiredoxins)
      0 (Antioxidants)
      EC 1.11.1.9 (Glutathione Peroxidase)
      EC 1.11.1.6 (Catalase)
      BBX060AN9V (Hydrogen Peroxide)
      EC 1.11.1.15 (PRDX2 protein, human)
    • Publication Date:
      Date Created: 20240816 Date Completed: 20240910 Latest Revision: 20241101
    • Publication Date:
      20241101
    • Accession Number:
      10.1016/j.abb.2024.110121
    • Accession Number:
      39151873