microRNA profiling of exosomes derived from plasma and their potential as biomarkers for Opisthorchis viverrini-associated cholangiocarcinoma.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6254 (Electronic) Linking ISSN: 0001706X NLM ISO Abbreviation: Acta Trop Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Basel.
    • Subject Terms:
      Cholangiocarcinoma*/parasitology ; Cholangiocarcinoma*/blood ; Cholangiocarcinoma*/genetics ; Cholangiocarcinoma*/diagnosis ; MicroRNAs*/blood ; MicroRNAs*/genetics ; Exosomes*/genetics ; Opisthorchis*/genetics ; Opisthorchiasis*/complications ; Opisthorchiasis*/parasitology ; Opisthorchiasis*/blood ; Opisthorchiasis*/diagnosis ; Bile Duct Neoplasms*/parasitology ; Bile Duct Neoplasms*/blood ; Bile Duct Neoplasms*/genetics ; Bile Duct Neoplasms*/diagnosis; Animals ; Humans ; Biomarkers, Tumor/blood ; Biomarkers, Tumor/genetics ; Male ; Middle Aged ; Female ; High-Throughput Nucleotide Sequencing ; Gene Expression Profiling ; Computational Biology/methods ; Aged
    • Abstract:
      Cholangiocarcinoma (CCA) is a life-threatening disease that impacts patients worldwide. In Southeast Asian countries, the liver fluke Opisthorchis viverrini plays a major role in inducing carcinogenesis of the bile ducts. Due to its asymptomatic nature, O. viverrini infections are rarely treated, consequently leading to the development of advanced stages of CCA before diagnosis. Despite the current use of exosomal microRNAs (miRNA) as diagnostic biomarkers for the early detection of many types of cancer, the applications for miRNA remain limited with CCA. Circulating exosomes, membranous vesicles essential for intercellular communication, were found to contain unique miRNA. In this study, we conducted next-generation sequencing (Ion Torrent PGM) and bioinformatics to characterize and compare the contents of exosomal miRNA derived from the plasma of CCA patients, O. viverrini-infected patients, and healthy individuals, as well as to identify and validate key molecules as markers for screening the diagnosis of CCA and O. viverrini infection. The obtained results showed the success of using NGS technology in discovering exosomal miRNAs, specifically miR-194-5p and miR-192-5p, both of which were upregulated in the O. viverrini-infected group. Interestingly, miR-192-5p was upregulated while miR-194-5p was downregulated in CCA, suggesting their potential use as biomarkers for screening CCA and O. viverrini infection, especially in O. viverrini-endemic areas.
      Competing Interests: Declaration of competing interest The authors declared no competing interests for this study
      (Copyright © 2024 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Biomarker; Cholangiocarcinoma; Exosome; Opisthorchis viverrini; miRNA
    • Accession Number:
      0 (MicroRNAs)
      0 (Biomarkers, Tumor)
    • Publication Date:
      Date Created: 20240816 Date Completed: 20240831 Latest Revision: 20240831
    • Publication Date:
      20240902
    • Accession Number:
      10.1016/j.actatropica.2024.107362
    • Accession Number:
      39151716