Proteomics and metabolomics analyses of urine for investigation of gallstone disease in a high-altitude area.

Publisher: Springer Country of Publication: United States NLM ID: 101274889 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-3890 (Electronic) Linking ISSN: 15733882 NLM ISO Abbreviation: Metabolomics Subsets: MEDLINE

Original Publication: New York : Springer, c2006-

Proteomics*/methods ; Metabolomics*/methods ; Gallstones*/metabolism ; Gallstones*/urine ; Biomarkers*/urine ; Altitude*; Humans ; Female ; Middle Aged ; Male ; Chromatography, Liquid/methods ; Adult ; Aged ; Mass Spectrometry/methods ; Case-Control Studies

Background: The incidence of gallstones is high in Qinghai Province. However, the molecular mechanisms underlying the development of gallstones remain unclear.
Methods: In this study, we collected urine samples from 30 patients with gallstones and 30 healthy controls. The urine samples were analysed using multi-omics platforms. Proteomics analysis was conducted using data-independent acquisition, whereas metabolomics analysis was performed using liquid chromatography-mass spectrometry (LC-MS).
Results: Among the patients with gallstones, we identified 49 down-regulated and 185 up-regulated differentially expressed proteins as well as 195 up-regulated and 189 down-regulated differentially expressed metabolites. Six pathways were significantly enriched: glycosaminoglycan degradation, arginine and proline metabolism, histidine metabolism, pantothenate and coenzyme A biosynthesis, drug metabolism-other enzymes, and the pentose phosphate pathway. Notably, 10 differentially expressed proteins and metabolites showed excellent predictive performance and were selected as potential biomarkers.
Conclusion: The findings of our metabolomics and proteomics analyses provide new insights into novel biomarkers for patients with cholelithiasis in high-altitude areas.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

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2023-SF-117 Qinghai Provincial Department of Science and Technology; Key discipline project in the School of Public Health(NO.21) Chengdu Medical College

Keywords: Gallstone; High altitude; Metabolomics; Proteomics

0 (Biomarkers)

Date Created: 20240814 Date Completed: 20240814 Latest Revision: 20241021

20241021

10.1007/s11306-024-02162-7

39143352