Effects of CRAMP on the gut-brain axis in experimental sepsis.

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  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7910006 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0542 (Electronic) Linking ISSN: 01652478 NLM ISO Abbreviation: Immunol Lett Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
    • Subject Terms:
      Sepsis*/immunology ; Sepsis*/etiology ; Sepsis*/metabolism ; Sepsis*/microbiology ; Mice, Knockout* ; Gastrointestinal Microbiome*/immunology ; Disease Models, Animal* ; Brain-Gut Axis* ; Cathelicidins*; Animals ; Mice ; Antimicrobial Cationic Peptides/metabolism ; Dysbiosis/immunology ; Male ; Mice, Inbred C57BL ; Brain/metabolism ; Brain/immunology
    • Abstract:
      The collaboration between the microbiota, mucosa, and intestinal epithelium is crucial for defending against pathogens and external antigens. Dysbiosis disrupts this balance, allowing pathogens to thrive and potentially enter the bloodstream, triggering immune dysregulation and potentially leading to sepsis. Antimicrobial peptides like LL-37 and CRAMP are pivotal in innate immune defense. Their expression varies with infection severity, exhibiting a dual pro- and anti-inflammatory response. Understanding this dynamic is key to comprehending sepsis progression. In our study, we examined the inflammatory response in CRAMP knockout mice post-cecal ligation and puncture (CLP). We assessed its impact on brain tissue damage and the intestinal microbiota. Our findings revealed higher gene expression of S100A8 and S100A9 in the prefrontal cortex of wild-type mice versus CRAMP-knockout mice. This trend was consistent in the hippocampus and cerebellum, although protein concentrations remained constant. Notably, there was a notable increase in Escherichia coli, Lactobacillus spp., and Enterococcus faecalis populations in wild-type mice 24 h post-CLP compared to the CRAMP-deficient group. These results align with our previous data suggesting that the absence of CRAMP may confer protection in this sepsis model.
      Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.
      (Copyright © 2024. Published by Elsevier B.V.)
    • Contributed Indexing:
      Keywords: Cathelicidins; Innate immunity; Microbiome; Neuroinflammation; Sepsis
    • Accession Number:
      0 (Cathelicidins)
      0 (Antimicrobial Cationic Peptides)
    • Publication Date:
      Date Created: 20240809 Date Completed: 20240907 Latest Revision: 20240907
    • Publication Date:
      20240908
    • Accession Number:
      10.1016/j.imlet.2024.106906
    • Accession Number:
      39122093