Comparison of the Effect of Different Treatment Doses of Intrastromal Vancomycin in a Rabbit Model of Methicillin-Resistant Staphylococcus Aureus (MRSA) Keratitis.

Publisher: Informa Healthcare Country of Publication: England NLM ID: 9312169 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1744-5078 (Electronic) Linking ISSN: 09273948 NLM ISO Abbreviation: Ocul Immunol Inflamm Subsets: MEDLINE

Publication: London : Informa Healthcare
Original Publication: Buren, The Netherlands : Aeolus Press, c1993-

Methicillin-Resistant Staphylococcus aureus*/isolation & purification ; Methicillin-Resistant Staphylococcus aureus*/drug effects ; Vancomycin*/administration & dosage ; Vancomycin*/therapeutic use ; Staphylococcal Infections*/drug therapy ; Staphylococcal Infections*/microbiology ; Eye Infections, Bacterial*/microbiology ; Eye Infections, Bacterial*/drug therapy ; Anti-Bacterial Agents*/administration & dosage ; Anti-Bacterial Agents*/therapeutic use ; Disease Models, Animal* ; Corneal Stroma*/drug effects ; Corneal Stroma*/microbiology; Animals ; Rabbits ; Dose-Response Relationship, Drug ; Corneal Ulcer/microbiology ; Corneal Ulcer/drug therapy ; Bacterial Load ; Keratitis/microbiology ; Keratitis/drug therapy ; Keratitis/diagnosis

Purpose: To compare the effect of different doses of vancomycin on a rabbit model of MRSA keratitis.
Methods: Twenty-four eyes of 24 New-Zealand White rabbits were included in the study. MRSA keratitis was applied to 24 left eyes of 24 New Zealand rabbits. Twenty-four hours after MRSA inoculation; 0.5 mg/0.1 mL, 1 mg/0.1 mL, and 2 mg/0.1 mL and balanced salt solution were administered to 6 rabbits in 4 groups, respectively.
Results: The effect of different doses of vancomycin on reducing bacterial load was found to be statistically significant when each was compared to the control group ( p  = 0.006). When comparing the dosages with each other, no superiority was shown ( p  = 0.297, p  = 0.749, p  = 0.262 respectively). There was a significant increase in the posttreatment total clinical score in the control and 2 mg/0.1 mL groups compared to the pretreatment score ( p  = 0.001, p  = 0.001 respectively).
Conclusion: It is emphasized that necessary treatment can be achieved by administering less antibiotic (0.5 mg/0.1 mL) to the corneal intrastromal area.

Keywords: Corneal intrastromal therapy; Methicillin-resistant Staphylococcus aureus(MRSA); fortified topical drop; keratitis; vancomycin

6Q205EH1VU (Vancomycin)
0 (Anti-Bacterial Agents)

Date Created: 20240808 Date Completed: 20241126 Latest Revision: 20241126

20241202

10.1080/09273948.2024.2382928

39115284