Chondroitin/dermatan sulphate proteoglycan, desmosealin, showing affinity to desmosomes.

Publisher: Blackwell Science Ltd Country of Publication: England NLM ID: 8007161 Publication Model: Print Cited Medium: Internet ISSN: 1468-2494 (Electronic) Linking ISSN: 01425463 NLM ISO Abbreviation: Int J Cosmet Sci Subsets: MEDLINE

Publication: 2000- : Oxford : Blackwell Science Ltd.
Original Publication: [Oxford, Blackwell Scientific Publications.]

Chondroitin*/metabolism ; Chondroitin Sulfate Proteoglycans*/metabolism ; Desmosomes*/metabolism; Humans

Objective: Desmosomes are the most prominent interkeratinocyte junctions. The correct barrier function of stratified epithelia such as epidermis depends on their expression. During epidermal differentiation, the molecular composition of desmosomes evolves and so do their physical and chemical properties. Desquamation of corneocytes at the surface of the stratum corneum depends on an orderly degradation of desmosomes by endogenous enzymes. This process may be regulated by glycosylated molecules. We focused on the detection and characterization of potentially implicated players bearing ‘sugar’ characteristics.
Methods: Using an original monoclonal antibody and biochemical methods, we partially characterized a proteoglycan of the exclusively chondroitin/dermatan sulphate type, secreted into the interkeratinocyte spaces, that is incorporated into the extracellular parts of desmosomes in quantities proportional to the degree of cell differentiation, as visualized with immuno-electron microscopy.
Results: This antigen, that we named desmosealin, displays biochemical and immunocytochemical characteristics that clearly differentiate it from known desmosomal elements. Unlike so far described epidermal proteoglycans, which belong to the heparan sulphate family, desmosealin displays chondroitin/dermatan sulphate glycosaminoglycan chains. It can be detected within the extracellular ‘cores’ of desmosomes in the upper viable epidermal layers and in corneodesmosomes from the lowermost part of the stratum corneum.
Conclusion: Extensive integration of proteoglycans into the extracellular parts of desmosomes at the late stages of keratinocyte maturation is likely of functional importance. Given its biochemical profile, its pattern of expression in the epidermis and its desmosomal localization, desmosealin may emerge as a key element in the regulation of desmosome processing, epidermal cohesion and formation of a functional epidermal barrier.
(© 2024 Society of Cosmetic Scientists and Societe Francaise de Cosmetologie.)

Erratum in: Int J Cosmet Sci. 2024 Sep 10. doi: 10.1111/ics.13026. (PMID: 39256194)

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Keywords: cell–cell junctions; differentiation; epidermis; intercellular matrix; skin barrier; skin physiology/structure
Local Abstract: [Publisher, French] Les desmosomes sont les jonctions inter‐kératinocytaires les plus proéminentes. Le fonctionnement appropriée des épithéliums stratifiés comme épiderme dépend de leur expression. La composition moléculaire et les propriétés physico‐chimiques des desmosomes évoluent au cours de la différenciation épidermique. La desquamation de cornéocytes la surface du stratum corneum depend de la dégradation ordonnée des desmosomes par les enzymes endogènes. Ce processus peut être régulé par les molécules glycosylées. Notre travail consistait en détection et caractérisation de l'un des acteurs potentiellement impliqués, portant des chaînes carbohydrate. [Publisher, French] Les approches d'analyse biochimique s'appuyant sur un anticorps monoclonal original (immunotransfert mono‐et bi‐dimensionnel, immunoprécipitation–immunodétection croisées, digestions enzymatiques, tests de déglycosylation et d'inhibition de synthèse) nous ont permis la caractérisation partielle d'un protéoglycanne sécrété dans les espaces inter‐kératinocytaires. Cette molécule s'intègre aux desmosomes en quantités proportionnelles au stade de différenciation des kératinocytes, comme le démontrent les marquages ultrastructuraux à l'or colloïdal sur des cryocoupes et tissus enrobés en résines acryliques. [Publisher, French] Cet antigène, que nous avons appelé desmosealine, est clairement distinct des éléments constitutifs de desmosomes décrits jusqu'alors. Contrairement aux protéoglycannes épidermiques connus, il porte exclusivement les chaînes glycosaminoglycannes de type chondroïtine/dermatane sulfate. La desmosealine est présente dans les parties extracellulaires de desmosomes, dans la portion supérieure de l‘épiderme vivant et le début du stratum corneum. [Publisher, French] L'intégration massive d'un protéoglycanne dans des parties intercellulaires de desmosomes revêt vraisemblablement une importance fonctionnelle. De par son profile biochimique, sa distribution dans l'épiderme et son affinité pour les desmosomes, le desmosealine peut s'avérer être un élément clé dans la régulation de la cohésion interkératinocytaire et la formation de la barrière de perméabilité épidermique.

9007-27-6 (Chondroitin)
0 (Chondroitin Sulfate Proteoglycans)

Date Created: 20240808 Date Completed: 20240808 Latest Revision: 20240910

20240911

10.1111/ics.12954

39113319