Current Protocols: Alopecia Areata Mouse Models for Drug Efficacy and Mechanism Studies.

Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 101773894 Publication Model: Print Cited Medium: Internet ISSN: 2691-1299 (Electronic) Linking ISSN: 26911299 NLM ISO Abbreviation: Curr Protoc Subsets: MEDLINE

Original Publication: Hoboken, NJ : John Wiley & Sons, [2021]-

Alopecia Areata*/drug therapy ; Alopecia Areata*/pathology ; Alopecia Areata*/immunology ; Disease Models, Animal* ; Mice, Inbred C3H* ; Skin Transplantation*; Animals ; Mice ; Female ; Male ; Adoptive Transfer

Alopecia areata is the second most common form of hair loss in humans after androgenetic alopecia. Although a variety of animal models for alopecia areata have been described, currently the C3H/HeJ mouse model is the most commonly used and accepted. Spontaneous hair loss occurs in 15%-25% of older mice in which the lesions wax and wane, similar to the human disease, with alopecia being more common and severe in female mice. Full-thickness skin grafts from mice with spontaneous alopecia areata to young, normal-haired, histocompatible mice provide a highly reproducible model with progressive lesions that makes it useful for drug efficacy and mechanism-based studies. As alopecia areata is a cell-mediated autoimmune disease, transfer of cultured lymph node cells from affected mice to unaffected, histocompatible recipients also promotes disease development and provides an alternative, nonsurgical protocol. Protocols are presented to produce these models such that they can be used to study alopecia areata and to develop novel drug therapies. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Full-thickness skin grafts to reproducibly induce alopecia areata in C3H/HeJ mice Basic Protocol 2: Adoptive transfer of cultured lymphoid cells provides a nonsurgical method to induce alopecia areata in C3H/HeJ mice.
(© 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.)

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The National Alopecia Areata Foundation

Keywords: alopecia areata; cell transplants; drug efficacy trials; full‐thickness skin grafts; mouse models

Date Created: 20240806 Date Completed: 20240806 Latest Revision: 20240806

20240806

10.1002/cpz1.1113

39105684