[Concordance of DNA fragmentation times for next-generation sequencing library construction based on DNA electrophoresis rating method and Nano/Qubit ratio method].

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Gao J;Gao J; Liu TQ; Liu TQ; Lu T; Lu T
  • Source:
    Zhonghua bing li xue za zhi = Chinese journal of pathology [Zhonghua Bing Li Xue Za Zhi] 2024 Aug 08; Vol. 53 (8), pp. 855-858.
  • Publication Type:
    Journal Article
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Zhonghua Yixuehui Country of Publication: China NLM ID: 0005331 Publication Model: Print Cited Medium: Print ISSN: 0529-5807 (Print) Linking ISSN: 05295807 NLM ISO Abbreviation: Zhonghua Bing Li Xue Za Zhi Subsets: MEDLINE
    • Publication Information:
      Original Publication: Beijing : Zhonghua Yixuehui
    • Subject Terms:
      DNA Fragmentation* ; High-Throughput Nucleotide Sequencing*/methods ; Gene Library* ; DNA*; Humans ; Electrophoresis/methods ; Sequence Analysis, DNA/methods ; Time Factors
    • Contributed Indexing:
      Local Abstract: [Publisher, Chinese] 目的： 评价电泳评级法及Nano/Qubit浓度比值法在二代测序建库DNA片段化过程中超声打断时间的一致性。 方法： 收集中国医学科学院 北京协和医学院 北京协和医院病理科323例石蜡固定样本。分别通过DNA电泳评级法和Nano/Qubit浓度比值法确定二代测序建库DNA片段化过程中的超声打断时间，利用 Kappa 一致性检验对二者的一致性进行比较。 结果： Kappa 系数表明电泳评级法与Nano/Qubit浓度比值法2种方法评估DNA超声打断时间的一致性良好（ Kappa =0.540， P <0.05），但对于低质量标本，缺乏一致性：Nano/Qubit值>7.5（ Kappa =0.021， P =0.751）或D级（ Kappa =0.040， P =0.146）。 结论： 电泳评级法与Nano/Qubit浓度比值评估二代测序文库DNA片段化时间的一致性良好，可以用Nano/Qubit值大致推算标本的质量，但对于Nano/Qubit值>7.5的标本，应当通过电泳评级法进行评估。.
    • Accession Number:
      9007-49-2 (DNA)
    • Publication Date:
      Date Created: 20240805 Date Completed: 20240805 Latest Revision: 20240805
    • Publication Date:
      20240806
    • Accession Number:
      10.3760/cma.j.cn112151-20231211-00402
    • Accession Number:
      39103271