STOP1 regulates CCX1-mediated Ca ²⁺ homeostasis for plant adaptation to Ca ²⁺ deprivation.

Publisher: Wiley-Blackwell Pub Country of Publication: China (Republic : 1949- ) NLM ID: 101250502 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1744-7909 (Electronic) Linking ISSN: 16729072 NLM ISO Abbreviation: J Integr Plant Biol Subsets: MEDLINE

Publication: [China] : Wiley-Blackwell Pub
Original Publication: [Carlton South, Victoria] : Blackwell Pub., 2005-

Arabidopsis*/genetics ; Arabidopsis*/metabolism ; Arabidopsis*/physiology ; Arabidopsis Proteins*/metabolism ; Arabidopsis Proteins*/genetics ; Homeostasis* ; Calcium*/metabolism ; Gene Expression Regulation, Plant* ; Transcription Factors*/metabolism ; Transcription Factors*/genetics ; Adaptation, Physiological*/genetics; Mutation/genetics ; Promoter Regions, Genetic/genetics

Calcium (Ca) is essential for plant growth and stress adaptation, yet its availability is often limited in acidic soils, posing a major threat to crop production. Understanding the intricate mechanisms orchestrating plant adaptation to Ca deficiency remains elusive. Here, we show that the Ca deficiency-enhanced nuclear accumulation of the transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1) in Arabidopsis thaliana confers tolerance to Ca deprivation, with the global transcriptional responses triggered by Ca deprivation largely impaired in the stop1 mutant. Notably, STOP1 activates the Ca deprivation-induced expression of CATION/Ca ²⁺ EXCHANGER 1 (CCX1) by directly binding to its promoter region, which facilitates Ca ²⁺ efflux from endoplasmic reticulum to cytosol to maintain Ca homeostasis. Consequently, the constitutive expression of CCX1 in the stop1 mutant partially rescues the Ca deficiency phenotype by increasing Ca content in the shoots. These findings uncover the pivotal role of the STOP1-CCX1 axis in plant adaptation to low Ca, offering alternative manipulating strategies to improve plant Ca nutrition in acidic soils and extending our understanding of the multifaceted role of STOP1.
(© 2024 Institute of Botany, Chinese Academy of Sciences.)

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GZC20232327 Postdoctoral Fellowship Program of CPSF; Agricultural Science and Technology Innovation Program; 2023YFD1901800 National Key Research and Development Program of China; 2023YFD1902905 National Key Research and Development Program of China; LY23C130005 Natural Science Foundation of Zhejiang Province; 32201702 National Natural Science Foundation of China; Y2023QC21 Central Public-interest Scientific Institution Basal Research Fund

Keywords: CCX1; Ca deficiency; Ca homeostasis; STOP1; transcriptional response

0 (Arabidopsis Proteins)
SY7Q814VUP (Calcium)
0 (Transcription Factors)
0 (STOP1 protein, Arabidopsis)

Date Created: 20240802 Date Completed: 20241018 Latest Revision: 20241018

20241019

10.1111/jipb.13754

39092784