Hepatoprotective effect of astaxanthin against cholestasis liver fibrosis induced by bile duct ligation in adult Wistar rats.

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  • Additional Information
    • Source:
      Publisher: Wiley Country of Publication: United States NLM ID: 9717231 Publication Model: Print Cited Medium: Internet ISSN: 1099-0461 (Electronic) Linking ISSN: 10956670 NLM ISO Abbreviation: J Biochem Mol Toxicol Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, NY : Wiley, c1998-
    • Subject Terms:
      Xanthophylls*/pharmacology ; Xanthophylls*/therapeutic use ; Rats, Wistar* ; Cholestasis*/pathology ; Cholestasis*/metabolism ; Cholestasis*/drug therapy ; Liver Cirrhosis*/metabolism ; Liver Cirrhosis*/pathology ; Liver Cirrhosis*/drug therapy ; Liver Cirrhosis*/prevention & control ; Bile Ducts*/surgery; Animals ; Male ; Rats ; Ligation ; Liver/drug effects ; Liver/pathology ; Liver/metabolism ; Transforming Growth Factor beta1/metabolism
    • Abstract:
      In this study, we evaluated the hepatoprotective effects of astaxanthin, a natural carotenoid, against the cholestatic liver fibrosis induced by bile duct ligation (BDL). Toward this end, male rats were subjected to BDL and treated with astaxanthin for 35 days. Afterwards, their serum and liver biochemical factors were assessed. Also, histopathological and immunohistochemical analyses were performed to determine the fibrosis and the expression levels of alpha-smooth muscle actin (α-SMA) and transforming growth factor beta (TGF-ß1) in the liver tissue. Based on the results, BDL caused a significant increase in liver enzyme levels, blood lipids, and bilirubin, while decreasing the activity of superoxide dismutase(SOD), catalase (CAT), and glutathione (GSH) enzymes. Also, in the BDL rats, hepatocyte necrosis, infiltration of inflammatory lymphocytes, and hyperplasia of bile ducts were detected, along with a significant increase in α-SMA and TGF-ß1 expression. Astaxanthin, however, significantly prevented the BDL's detrimental effects. In all, 10 mg/kg of this drug maintained the bilirubin and cholesterol serum levels of BDL rats at normal levels. It also reduced the liver enzymes' activity and serum lipids, while increasing the SOD, CAT, and GSH activity in BDL rats. The expression of α-SMA and TGF-ß1 in the BDL rats treated with 10 mg/kg of astaxanthin was moderate (in 34%-66% of cells) and no considerable cholestatic fibrosis was observed in this group. However, administrating the 20 mg/kg of astaxanthin was not effective in this regard. These findings showed that astaxanthin could considerably protect the liver from cholestatic damage by improving the biochemical features and regulating the expression of related proteins.
      (© 2024 Wiley Periodicals LLC.)
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    • Contributed Indexing:
      Keywords: TGF‐ß1; astaxanthin; bile duct ligation; liver fibrosis; α‐SMA
    • Accession Number:
      8XPW32PR7I (astaxanthine)
      0 (Xanthophylls)
      0 (Transforming Growth Factor beta1)
      0 (Tgfb1 protein, rat)
    • Publication Date:
      Date Created: 20240801 Date Completed: 20240801 Latest Revision: 20240801
    • Publication Date:
      20240801
    • Accession Number:
      10.1002/jbt.23788
    • Accession Number:
      39087918