Single-Cell Analysis Reveals That CD47 mRNA Expression Correlates with Immune Cell Activation, Antiviral Isgs, and Cytotoxicity.

Publisher: Cell Physiol Biochem Press GmbH & Co KG Country of Publication: Germany NLM ID: 9113221 Publication Model: Print Cited Medium: Internet ISSN: 1421-9778 (Electronic) Linking ISSN: 10158987 NLM ISO Abbreviation: Cell Physiol Biochem Subsets: MEDLINE

Publication: 2019- : Düsseldorf, Germany : Cell Physiol Biochem Press GmbH & Co KG
Original Publication: Basel ; New York : S. Karger, 1991-2018.

CD47 Antigen*/metabolism ; CD47 Antigen*/genetics ; CD8-Positive T-Lymphocytes*/metabolism ; CD8-Positive T-Lymphocytes*/immunology ; Single-Cell Analysis* ; Granzymes*/metabolism ; Granzymes*/genetics ; Killer Cells, Natural*/immunology ; Killer Cells, Natural*/metabolism ; Perforin*/metabolism ; Perforin*/genetics ; HIV-1*/immunology ; RNA, Messenger*/metabolism ; RNA, Messenger*/genetics ; Programmed Cell Death 1 Receptor*/metabolism ; Programmed Cell Death 1 Receptor*/genetics; Humans ; COVID-19/immunology ; COVID-19/virology ; COVID-19/genetics ; HIV Infections/immunology ; HIV Infections/virology ; HIV Infections/genetics ; Lymphocyte Activation Gene 3 Protein ; Antigens, Differentiation, T-Lymphocyte/metabolism ; Antigens, Differentiation, T-Lymphocyte/genetics ; SARS-CoV-2/immunology ; Interferon-gamma/metabolism ; Interferon-gamma/genetics ; Monocytes/metabolism ; Monocytes/immunology ; Antigens, CD/metabolism ; Antigens, CD/genetics ; B-Lymphocytes/metabolism ; B-Lymphocytes/immunology ; Immunity, Innate

Background/aims: Immune cells are reported to upregulate CD47 during infection, however, the role of CD47 in innate and adaptive immune cells remains unclear.
Methods: To bridge this knowledge gap, we analysed our single cell (sc)-RNA dataset along with other publicly available sc-RNA datasets from healthy controls, people with HIV-1 (PWH) and COVID-19 patients. We characterized each immune cell based on low, intermediate, and high expression of CD47 .
Results: Our analyses revealed that CD47 ^high pDCs and monocytes exhibited relatively higher expression of IFN-α regulatory genes, antiviral interferon-stimulated genes (ISGs) and MHC-I associated genes compared to CD47 ^inter. and CD47 ^low cells. Furthermore, CD47 ^high NK and CD8+ T cells showed higher expression of antiviral ISGs, as well as genes encoding for cytotoxic markers like granzyme B, perforin, granulysin, interferon gamma and NKG7. Additionally, CD47 ^high CD8+ T cells expressed higher levels of PD-1 and LAG-3 genes. Lastly, we found that CD47 ^high B cells had enriched expression of genes involved in cell activation and humoral responses.
Conclusion: Overall, our analyses revealed that innate and adaptive immune cells expressing elevated activation and functional gene signatures also express higher CD47 levels.
Competing Interests: The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors have declared that no competing interest exists.
(© Copyright by the Author(s). Published by Cell Physiol Biochem Press.)

R313-2019-790 Denmark The Lundbeck Foundation

Keywords: CD47 ; Immune cells ; Transcriptomic ; Infection ; Antiviral ; Cytotoxic

0 (CD47 Antigen)
EC 3.4.21.- (Granzymes)
126465-35-8 (Perforin)
0 (RNA, Messenger)
0 (Programmed Cell Death 1 Receptor)
0 (CD47 protein, human)
0 (PDCD1 protein, human)
0 (Lymphocyte Activation Gene 3 Protein)
0 (GNLY protein, human)
0 (Antigens, Differentiation, T-Lymphocyte)
EC 3.4.21.- (GZMB protein, human)
82115-62-6 (Interferon-gamma)
0 (Antigens, CD)

Date Created: 20240729 Date Completed: 20240729 Latest Revision: 20240729

20240730

10.33594/000000715

39074350