Novel shish-kebab structured nanofibrous decorating chitosan unidirectional scaffolds to mimic extracellular matrix for tissue engineering.

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  • Author(s): Feng PY;Feng PY; Jing X; Jing X
  • Source:
    Journal of the mechanical behavior of biomedical materials [J Mech Behav Biomed Mater] 2024 Oct; Vol. 158, pp. 106677. Date of Electronic Publication: 2024 Jul 25.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101322406 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-0180 (Electronic) Linking ISSN: 18780180 NLM ISO Abbreviation: J Mech Behav Biomed Mater Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam : Elsevier
    • Subject Terms:
      Chitosan*/chemistry ; Tissue Engineering* ; Tissue Scaffolds*/chemistry ; Nanofibers*/chemistry ; Extracellular Matrix*/metabolism ; Extracellular Matrix*/chemistry ; Polyesters*/chemistry; Mice ; Animals ; Porosity ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Biomimetic Materials/chemistry ; 3T3 Cells ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology
    • Abstract:
      Electrospun nanofibrous scaffolds are renowned for their ability to mimic the microstructure of the extracellular matrix (ECM). However, they often fail to replicate the geometry of target tissues, and the biocompatibility of these scaffolds those made from synthetic polymers is always limited due to the lack of cell binding sites. To address these issues, we proposed an innovative approach that combined unidirectional freeze-drying and electrospinning. During this process, electrospun polycaprolactone (PCL) nanofibers were chopped into nanofibrils, which range in size up to several hundred micrometers, and were incorporated into the chitosan scaffolds via unidirectional freeze-drying. In these scaffolds, the chitosan phase was responsible for maintaining the structural integrity at the macroscale, while the embedded nanofibers enhanced the surface topography at the microscale. The resulting scaffolds exhibited a high porosity of 90% and an impressive water uptake capacity of 2500%. Furthermore, 3T3 fibroblast cells showed strong interactions with the scaffolds, characterized by high rates of cell proliferation and viability. The cells also displayed significant orientation along the direction of the pores, suggesting that the scaffolds effectively guided cellular growth.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: High porosity; Tissue engineering; Topography; Unidirectional freeze-drying
    • Accession Number:
      9012-76-4 (Chitosan)
      24980-41-4 (polycaprolactone)
      0 (Polyesters)
      0 (Biocompatible Materials)
    • Publication Date:
      Date Created: 20240728 Date Completed: 20240827 Latest Revision: 20240827
    • Publication Date:
      20240828
    • Accession Number:
      10.1016/j.jmbbm.2024.106677
    • Accession Number:
      39068847