L-arginine attenuates dichlorvos-induced testicular toxicity in male Wistar rats by suppressing oxidative stress-dependent activation of caspase 3-mediated apoptosis.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Editions Scientifiques Elsevier Country of Publication: France NLM ID: 8213295 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1950-6007 (Electronic) Linking ISSN: 07533322 NLM ISO Abbreviation: Biomed Pharmacother Subsets: MEDLINE
    • Publication Information:
      Publication: Paris : Editions Scientifiques Elsevier
      Original Publication: New York, N.Y. : Masson Pub. USA, Inc., c1982-
    • Subject Terms:
      Apoptosis*/drug effects ; Arginine*/pharmacology ; Caspase 3*/metabolism ; Dichlorvos*/toxicity ; Oxidative Stress*/drug effects ; Testis*/drug effects ; Testis*/pathology ; Testis*/metabolism; Animals ; Male ; Rats ; Antioxidants/pharmacology ; Nitric Oxide/metabolism ; Rats, Wistar ; Sperm Count ; Spermatozoa/drug effects ; Testosterone/blood
    • Abstract:
      Background: The continuous use of pesticides, such as dichlorvos, is a common agricultural and domestic practice. However, it is associated with shortfalls like testicular toxicity through the induction of oxidative stress-mediated signaling. On the other hand, L-arginine, a precursor of nitric oxide, has been reported to exert antioxidant activities and thus may attenuate dichlorvos-induced testicular toxicity.
      Aim: Hence, this study was designed to evaluate the effect of L-arginine treatment on dichlorvos-induced testicular toxicity.
      Materials and Methods: Forty male Wistar rats were randomly assigned into four equal groups. The control rats were administered 0.5 mL of distilled water, dichlorvos- (DDVP-) treated rats were exposed to DDVP via inhalation for 15 min, DDVP + L-arginine-treated rats were exposed to DDVP and also received 100 mg/kg b.w/day, while L-arginine-treated rats received 100 mg/kg b.w/day.
      Results: DDVP exposure significantly reduced testicular nitric oxide, relative testicular weight, lowered sperm count, viability, and motility, and suppressed serum FSH, LH, and testosterone levels. These findings were associated with a rise in testicular malondialdehyde, TNF-α, IL-6, and 8OHdG levels and caspase 3 activities, and a reduction in GSH and superoxide dismutase. Additionally, on histopathological examination, DDVP was observed to reduce mature sperm cells in the seminiferous tubular lumen and induce focal vascular congestion in the interstitial space. Nonetheless, L-arginine treatment significantly attenuated DDVP-induced biochemical and histological alterations.
      Conclusion: This study showed that L-arginine attenuated testicular toxicity by improving epididymal sperm variables and male sex hormones by suppressing oxidative stress, inflammation, and apoptosis in DDVP-exposed rats.
      Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
    • Contributed Indexing:
      Keywords: Apoptosis; Dichlorvos; L-arginine; Oxidative stress; Pesticides; Spermatozoa
    • Accession Number:
      0 (Antioxidants)
      94ZLA3W45F (Arginine)
      EC 3.4.22.- (Caspase 3)
      7U370BPS14 (Dichlorvos)
      31C4KY9ESH (Nitric Oxide)
      3XMK78S47O (Testosterone)
    • Publication Date:
      Date Created: 20240727 Date Completed: 20240824 Latest Revision: 20240826
    • Publication Date:
      20240827
    • Accession Number:
      10.1016/j.biopha.2024.117136
    • Accession Number:
      39067166