A New Dual Fluorescence Method for Rapid Detection of Infectious Bronchitis Virus at Constant Temperature.

Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101625893 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2607 (Print) Linking ISSN: 20762607 NLM ISO Abbreviation: Microorganisms Subsets: PubMed not MEDLINE

Original Publication: Basel, Switzerland : MDPI AG, [2013]-

Infectious bronchitis virus (IBV) causes infectious bronchitis in chicken, an acute, highly contagious respiratory infection. Because of genetic mutations and recombination, IBV forms many subtypes, which makes it difficult to treat the disease and apply commercial vaccines. Therefore, to detect IBV in time and stop the virus from spreading, a novel and convenient IBV detection technology based on reverse transcription recombinase-aided amplification (RT-RAA) was established in this study. According to the S1 gene of IBV CH I-V and Mass genotypes and S1 gene of IBV CH VI genotype, a set of optimal primers were designed and selected to establish a real-time dual fluorescence RT-RAA method. The lowest detection line was 10 copies/μL of RNA molecules and the method exhibited no cross-reactivity with avian reticuloendotheliosis virus (REV), infectious bursal disease virus (IBDV), avian leukosis virus (ALV), Newcastle disease virus (NDV), chicken infectious anemia virus (CIAV), infectious laryngotracheitis virus (ILTV), Marek's disease virus (MDV), and H9N2 avian influenza virus (H9N2), demonstrating high specificity. When compared to qPCR detection results, our method achieved a sensitivity of 96.67%, a specificity of 90%, and a Kappa value of 0.87 for the IBV CH I-V and Mass genotypes, and achieved a sensitivity of 100%, a specificity of 97.73%, and a Kappa value of 0.91 for the IBV CH VI genotype.

Sci Rep. 2017 Jul 26;7(1):6576. (PMID: 28747730)
Vet Microbiol. 2010 Jul 14;143(2-4):145-54. (PMID: 20022714)
Virol J. 2015 Aug 04;12:116. (PMID: 26239707)
Poult Sci. 2019 Nov 1;98(11):5374-5384. (PMID: 31264704)
Mol Cell Probes. 2021 Dec;60:101777. (PMID: 34737039)
Virus Genes. 2012 Feb;44(1):19-23. (PMID: 21842233)
Avian Dis. 2022 Jun;66(2):135-140. (PMID: 35510473)
Virol J. 2022 Jul 30;19(1):129. (PMID: 35907986)
Int J Mol Sci. 2022 Dec 03;23(23):. (PMID: 36499594)
Proc Natl Acad Sci India Sect B Biol Sci. 2015;85(3):815-820. (PMID: 32226206)
J Vet Med Sci. 2018 Apr 27;80(4):725-730. (PMID: 29491226)
Avian Dis. 2020 Mar;64(1):80-84. (PMID: 32267128)
Dev Comp Immunol. 2000 Mar-Apr;24(2-3):187-200. (PMID: 10717287)
Virus Genes. 1995 Feb;9(3):219-29. (PMID: 7597801)
Onderstepoort J Vet Res. 2014 Sep 09;81(1):. (PMID: 25685887)
J Virol Methods. 2021 Oct;296:114227. (PMID: 34224752)
Avian Pathol. 2012;41(3):239-50. (PMID: 22702451)
Virol J. 2011 Apr 22;8:184. (PMID: 21510909)
J Integr Agric. 2016 Sep;15(9):2105-2113. (PMID: 32288952)
Poult Sci. 2022 Mar;101(3):101707. (PMID: 35108659)
Vaccine. 2015 Feb 25;33(9):1113-20. (PMID: 25636916)
Poult Sci. 1976 Jul;55(4):1482-5. (PMID: 181740)
Transbound Emerg Dis. 2022 Jul;69(4):2266-2274. (PMID: 34273259)
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2019 Oct 16;31(5):468-473. (PMID: 31713373)
J Microbiol Methods. 2017 Aug;139:202-204. (PMID: 28619662)
Transbound Emerg Dis. 2020 Mar;67(2):877-883. (PMID: 31714018)
Arch Virol. 2006 Jun;151(6):1133-48. (PMID: 16397751)

DT20220003 the Heyuan Branch, Guangdong Laboratory for Lingnan Modern Agriculture Project; 2023A1515010584 the Guangdong Basic and Applied Basic Research Foundation; CARS-42-13 China Agriculture Research System of MOF and MARA

Keywords: detection at constant temperature; infectious bronchitis virus; recombinase-aided amplification

Date Created: 20240727 Latest Revision: 20240729

20240729

PMC11279307

10.3390/microorganisms12071315

39065085